Haig D M, Blackie W, Huntley J, Mackellar A, Smith W D
Moredun Research Institute, Edinburgh, U.K.
Immunology. 1988 Oct;65(2):199-203.
Ovine mast cells generated in vitro are morphologically and biochemically similar to mast cells localized to the gastrointestinal tract in vivo. Bone marrow (BM)-derived haemopoietic precursor cells were cultured in the presence of conditioned medium (CM) prepared from in vitro-activated lymphocytes from nematode-infected sheep. Mast cells developed rapidly after 2 weeks of culture and could persist for 2-3 months. The presence of sheep mast cell proteinase (SMCP) in the cultured cells correlated with the number of morphologically recognizable mast cells by Leishman-Giemsa staining and is definitive as, in vivo, this enzyme is unique to mast cells. The content of SMCP per cell increased as the cultures developed. In vivo, increased serum concentrations of SMCP are associated with mast cell function at the site of nematode infection. The availability of the cultured cells should now facilitate mast cell biochemical and functional studies.
体外培养产生的绵羊肥大细胞在形态和生化方面与体内定位于胃肠道的肥大细胞相似。将来自骨髓（BM）的造血前体细胞在由线虫感染绵羊的体外活化淋巴细胞制备的条件培养基（CM）存在下进行培养。培养2周后肥大细胞迅速发育，并可持续2 - 3个月。培养细胞中绵羊肥大细胞蛋白酶（SMCP）的存在与经利什曼 - 吉姆萨染色在形态上可识别的肥大细胞数量相关，并且是确定的，因为在体内，这种酶是肥大细胞特有的。随着培养的进行，每个细胞的SMCP含量增加。在体内，血清中SMCP浓度的增加与线虫感染部位的肥大细胞功能相关。现在，培养细胞的可得性应有助于肥大细胞的生化和功能研究。
