Ifremer, Fisheries Research Laboratory, L'Houmeau 17137, France; MARBEC, Ifremer, Université de Montpellier, CNRS, IRD, Palavas-les-flots, France.
Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK.
Physiol Behav. 2020 Mar 15;216:112801. doi: 10.1016/j.physbeh.2020.112801. Epub 2020 Jan 11.
Individual stress coping style (reactive, intermediate and proactive) was determined in 3 groups of 120 pit tagged European seabass using the hypoxia avoidance test. The same three groups (no change in social composition) were then reared according to the standards recommended for this species. Then, 127 days later, individuals initially characterized as reactive, intermediate or proactive were submitted to an acute confinement stress for 30 min. Blood samples were taken to measure plasma cortisol levels 30 min (Stress30) or 150 min (Stress150) after the end of the confinement stress. Individuals were then sacrificed to sample the telencephalon in order to measure the main monoamines and their catabolites (at Stress30 only). Individuals from Stress150 were sampled for whole brain for a transcriptomic analysis. The main results showed that reactive individuals had a lower body mass than intermediate individuals which did not differ from proactive individuals. The physiological cortisol response did not differ between coping style at Stress30 but at Stress150 when intermediate and proactive individuals had recovered pre stress levels, reactive individuals showed a significant higher level illustrating a modulation of stress recovery by coping style. Serotonin turnover ratio was higher in proactive and reactive individuals compared to intermediate individuals and a significant positive correlation was observed with cortisol levels whatever the coping style. Further, the confinement stress led to a general increase in the serotonin turnover comparable between coping styles. Stress150 had a significant effect on target mRNA copy number (Gapdh mRNA copy number decreased while ifrd1 mRNA copy number increased) and such changes tended to depend upon coping style.
采用回避缺氧测试，在 3 组 120 尾标记有鲈鱼中确定了个体的应激应对风格（反应型、中间型和主动型）。然后，根据该物种推荐的标准，在不改变社会组成的情况下，将这三组（无社会组成变化）重新饲养。127 天后，最初被归类为反应型、中间型或主动型的个体经历了 30 分钟的急性限制应激。在限制应激结束后 30 分钟（Stress30）或 150 分钟（Stress150）取血样测量血浆皮质醇水平。然后将个体处死，以取样端脑，测量主要单胺及其代谢产物（仅在 Stress30 时）。来自 Stress150 的个体被取样用于全脑转录组分析。主要结果表明，反应型个体的体重低于中间型个体，而中间型个体与主动型个体没有差异。在 Stress30 时，应对方式之间的生理皮质醇反应没有差异，但在 Stress150 时，中间型和主动型个体已经恢复到应激前水平，而反应型个体则表现出显著更高的水平，表明应对方式对应激恢复有调节作用。与中间型个体相比，主动型和反应型个体的血清素周转率更高，并且无论应对方式如何，与皮质醇水平呈显著正相关。此外，限制应激导致所有应对方式之间的血清素周转率普遍增加。Stress150 对靶 mRNA 拷贝数有显著影响（Gapdh mRNA 拷贝数减少，而 ifrd1 mRNA 拷贝数增加），这种变化似乎取决于应对方式。
