College of Horticulture, Qingdao Agricultural University, Qingdao, 266109, China.
Curr Microbiol. 2020 May;77(5):855-864. doi: 10.1007/s00284-020-01875-x. Epub 2020 Jan 13.
Fusarium oxysporum f. sp. radicis-lycopersici (Forl) causes Fusarium crown and root rot of tomato, leading to severe yield losses. Chinese chive and the Chinese chive extract reportedly have antifungal effects. In this study, Chinese chive extract treatments inhibited Forl spore germination, with an EC of 0.40 g ml in vitro. Furthermore, the mechanism underlying the fungicidal effects of the Chinese chive extract was analyzed by RNA sequencing. A total of 1252 differentially expressed genes (DEGs) were detected, of which 396 were upregulated and 856 were downregulated. The DEGs were related to starch and sucrose metabolism, amino sugar and nucleotide sugar metabolism, galactose metabolism, fatty acid metabolism, sphingolipid metabolism, glycerophospholipid metabolism, peroxisomes, ribosome biogenesis in eukaryotes, mismatch repair, and the phosphatidylinositol signaling system, implying these pathways contribute to the fungicidal activity of the Chinese chive extract. The qRT-PCR results verified the accuracy of the RNA sequencing data. Thus, the Chinese chive extract can inhibit Forl spore germination by affecting spore nutrient metabolism.
尖镰孢茄病镰孢菌（Forl）引起番茄枯萎病和根腐病，导致严重的产量损失。据报道，韭菜及其提取物具有抗真菌作用。在这项研究中，韭菜提取物处理抑制了 Forl 孢子的萌发，其在体外的 EC₅₀ 为 0.40 g/ml。此外，还通过 RNA 测序分析了韭菜提取物的杀菌作用机制。共检测到 1252 个差异表达基因（DEGs），其中 396 个上调，856 个下调。DEGs 与淀粉和蔗糖代谢、氨基糖和核苷酸糖代谢、半乳糖代谢、脂肪酸代谢、鞘脂代谢、甘油磷脂代谢、过氧化物酶体、真核生物核糖体生物发生、错配修复和磷脂酰肌醇信号系统有关，这表明这些途径有助于韭菜提取物的杀菌活性。qRT-PCR 结果验证了 RNA 测序数据的准确性。因此，韭菜提取物可以通过影响孢子营养代谢来抑制 Forl 孢子的萌发。
