Loria Antonio Di, Dattilo Vincenzo, Santoro Domenico, Guccione Jacopo, De Luca Adriana, Ciaramella Paolo, Pirozzi Marinella, Iaccino Enrico
Department of Veterinary Medicine and Animal Productions, University Federico II, 80130 Napoli, Italy.
Department of Health Sciences, Magna Graecia University, 88100 Catanzaro, Italy.
Animals (Basel). 2020 Jan 8;10(1):100. doi: 10.3390/ani10010100.
Current knowledge on the role of exosomal microRNA (miRNA) in canine leishmaniasis (CL), with particular regards to the interaction between miR-122 and lipid alterations, is limited. The aim of this study was to isolate/characterize exosomes in canine serum and evaluate the expression of miR-122 in ten healthy and ten leishmaniotic dogs. Serum exosomes were isolated using a polymer-based kit, ExoQuick and characterized by flow cytometry and transmission electron microscopy, whereas miR-122-5p expression was evaluated by quantitative reverse-transcriptase polymerase chain reaction. A significant decreased expression of exosomal miR-122-5p, decreased serum levels of high-density lipoproteins, and increased serum levels of low-density lipoproteins were seen in leishmaniotic dogs when compared with healthy dogs. These results suggest that hepatic dysfunctions induced by the parasite interfere with lipoprotein status. The decreased expression of exosomal miR122 represents an additional effect of infection in dogs as in people.
目前关于外泌体微小RNA（miRNA）在犬利什曼病（CL）中的作用，特别是miR-122与脂质改变之间相互作用的知识有限。本研究的目的是分离/表征犬血清中的外泌体，并评估10只健康犬和10只患利什曼病犬中miR-122的表达。使用基于聚合物的试剂盒ExoQuick分离血清外泌体，并通过流式细胞术和透射电子显微镜进行表征，而miR-122-5p的表达则通过定量逆转录聚合酶链反应进行评估。与健康犬相比，患利什曼病的犬中外泌体miR-122-5p表达显著降低，血清高密度脂蛋白水平降低，血清低密度脂蛋白水平升高。这些结果表明，寄生虫诱导的肝功能障碍会干扰脂蛋白状态。外泌体miR122表达降低代表了犬感染与人类感染一样的额外影响。
