Department of Biotechnology and Food-Technology, University of Johannesburg, 55 Beit Street, Doornfontein, Johannesburg 2028, South Africa.
DST-NRF Centre of Excellence for Biomedical Tuberculosis Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg Campus, Cape Town 7505, South Africa.
Int J Environ Res Public Health. 2020 Jan 9;17(2):439. doi: 10.3390/ijerph17020439.
Plant endophytes are microbial sources of bioactive secondary metabolites, which mimic the natural compounds chemistry of their respective host plants in a similar manner. This study explored the isolation and identification of fungal endophytes, and investigated the antibacterial and antimycobacterial activity of their crude extracts. Fungal endophytes were isolated from , identified using morphological traits and internal transcribed spacer ribosomal-deoxyribonucleic acid (ITS-rDNA) sequence analysis. Eight fungal endophytes were identified as , sp., sp., sp., and sp., while was isolated from the leaves and unripe fruits. Good activity was observed for the crude extracts of inhibiting , and at 6 µg/mL. Crude extracts of sp., showed activity at 9 μg/mL against and . In general, the crude extracts showed great activity against Gram-negative and Gram-positive bacteria and novel results for two mycobacteria species and . The results provide evidence of diverse fungal endophytes isolated from and evidence that fungal endophytes are a good source of bioactive compounds with pharmaceutical potential, particularly against
植物内生真菌是生物活性次生代谢物的微生物来源，它们以类似的方式模拟其宿主植物的天然化合物化学。本研究探索了内生真菌的分离和鉴定，并研究了其粗提物的抗菌和抗分枝杆菌活性。从内生真菌中分离出内生真菌，并用形态特征和内部转录间隔区核糖体脱氧核糖核酸 (ITS-rDNA) 序列分析进行鉴定。鉴定出的 8 种内生真菌分别为 、 、 、 、 、 、 和 ，而 是从叶片和未成熟果实中分离出来的。粗提取物对 6 µg/mL 的 、 和 有很好的抑制活性。 、 、 和 对 和 的 9 μg/mL 粗提取物表现出活性。总的来说，粗提取物对革兰氏阴性菌和革兰氏阳性菌具有很强的活性，对 和 两种分枝杆菌属的新型结果。结果为从 中分离出的不同内生真菌提供了证据，也为内生真菌是具有药物潜力的生物活性化合物的良好来源提供了证据，特别是针对
