Francis J L, el-Baruni K, Roath O S, Taylor I
University Department of Haematology, Southampton General Hospital, United Kingdom.
Thromb Res. 1988 Nov 1;52(3):207-17. doi: 10.1016/0049-3848(88)90080-1.
The factor X-activating activity (FXAA) of homogenates from human colorectal tumours and corresponding normal colonic mucosa from the same patients was assessed with a specific chromogenic substrate technique. FXAA was detected in all normal and tumour tissue tested, but was significantly higher in tumour tissue. The procoagulant activity was inhibited by DFP, but was unaffected by iodoacetamide and mercuric chloride. FXAA was largely abolished by prior incubation of both normal and tumour tissue homogenates with a rabbit anti-human factor VII serum, but was greatly enhanced by the addition of purified factor VII. FXAA was partially adsorbed on to aluminium hydroxide and almost completely abolished by treatment with barium citrate. It is concluded that the FXAA of both normal and malignant colorectal tissue is the result of tissue factor-factor VII interaction.
采用特异性显色底物技术评估了来自同一患者的人大肠肿瘤匀浆以及相应正常结肠黏膜的X因子激活活性（FXAA）。在所有检测的正常组织和肿瘤组织中均检测到FXAA，但肿瘤组织中的FXAA显著更高。促凝活性被二异丙基氟磷酸（DFP）抑制，但不受碘乙酰胺和氯化汞影响。正常组织和肿瘤组织匀浆预先与兔抗人因子VII血清孵育后，FXAA基本被消除，但加入纯化的因子VII后则显著增强。FXAA部分吸附于氢氧化铝，用柠檬酸钡处理后几乎完全消除。结论是，正常和恶性大肠组织的FXAA均为组织因子 - 因子VII相互作用的结果。
