el-Baruni K, Taylor I, Roath S, Francis J L
University Department of Haematology, Southampton General Hospital, U.K.
Hematol Oncol. 1990 Nov-Dec;8(6):323-32. doi: 10.1002/hon.2900080604.
Factor X-activating activity (FXAA) was determined by a chromogenic assay in normal and malignant breast tissue. FXAA was found in all tissue (n = 38) irrespective of pathology, and the activity of normal tissue was similar to that of tumours. FXAA correlated with tissue hemoglobin in normal breast (p less than 0.02) but not in tumours. FXAA was markedly reduced by aluminium hydroxide, barium citrate, anti-human factor VII, DFP, PMSF and phospholipase C, but was unaffected by iodoacetamide and mercuric chloride. It is concluded that FXAA is a serine protease with the properties of a tissue factor-factor VII complex. FXAA occurs in normal and malignant breast tissue, although the 'normal' activity may be an artefact of the homogenization process.
采用显色法在正常和恶性乳腺组织中测定X因子激活活性（FXAA）。在所有组织（n = 38）中均检测到FXAA，无论其病理状态如何，且正常组织的活性与肿瘤组织相似。在正常乳腺组织中，FXAA与组织血红蛋白相关（p < 0.02），但在肿瘤组织中无此相关性。FXAA可被氢氧化铝、柠檬酸钡、抗人因子VII、二异丙基氟磷酸（DFP）、苯甲基磺酰氟（PMSF）和磷脂酶C显著降低，但不受碘乙酰胺和氯化汞的影响。得出的结论是，FXAA是一种具有组织因子-因子VII复合物特性的丝氨酸蛋白酶。FXAA存在于正常和恶性乳腺组织中，尽管“正常”活性可能是匀浆过程中的人为产物。
