Dover R, Goeting N L, Taylor I, Roath O S, Francis J L
University Surgical Unit, Southampton General Hospital, UK.
Br J Surg. 1987 Dec;74(12):1122-4. doi: 10.1002/bjs.1800741216.
The ability of malignant tissue from 50 patients with colorectal carcinoma to activate blood coagulation factor X directly was compared with samples of adjacent, macroscopically normal colonic mucosa from the same patients, and tissue from four patients with non-malignant bowel disease. The resected tissue was homogenized and incubated with purified factor X and calcium ions. The subsequent generation of activated factor X was measured spectrophotometrically with a chromogenic substrate. Results were expressed as absorbance units, and as the ratio of tumour and normal activities. Factor X-activating activity (FXAA) was present in all normal and malignant tissues tested. FXAA was significantly greater (P less than 0.001) in the tumour homogenates than in the uninvolved tissue. The tumour:normal ratio was significantly (greater than 1.2) elevated in 38 patients (76 per cent). FXAA was not correlated with the degree of differentiation of the tumour, the Dukes' classification of the disease or the exact site of the tumour. There was no difference between the FXAA content of non-involved tissue from the colorectal cancer group and colonic mucosa from patients with non-malignant bowel disease. It is concluded that colorectal carcinomas contain significantly more FXAA than adjacent, non-malignant colonic mucosa from the same subject, but there is no direct evidence for a relationship between procoagulant levels and the extent of malignancy in these patients.
将50例结直肠癌患者的恶性组织直接激活凝血因子X的能力，与同一患者相邻的、肉眼可见正常的结肠黏膜样本以及4例非恶性肠道疾病患者的组织进行了比较。将切除的组织匀浆，并与纯化的因子X和钙离子一起孵育。随后用显色底物通过分光光度法测定活化因子X的生成量。结果以吸光度单位表示，并以肿瘤活性与正常活性的比值表示。在所有检测的正常和恶性组织中均存在因子X激活活性（FXAA）。肿瘤匀浆中的FXAA显著高于未受累组织（P小于0.001）。38例患者（76%）的肿瘤与正常组织的比值显著升高（大于1.2）。FXAA与肿瘤的分化程度、疾病的Dukes分期或肿瘤的确切部位无关。结直肠癌组未受累组织的FXAA含量与非恶性肠道疾病患者的结肠黏膜之间没有差异。结论是，结直肠癌所含的FXAA显著多于同一受试者相邻的非恶性结肠黏膜，但没有直接证据表明这些患者的促凝水平与恶性程度之间存在关联。
