Medical Biochemistry Department, National Research Centre, Dokki, Giza, Egypt.
Textile Research Division, National Research Centre, Dokki, Giza, Egypt.
Appl Biochem Biotechnol. 2020 Jul;191(3):1127-1139. doi: 10.1007/s12010-020-03230-z. Epub 2020 Jan 21.
Hyperglycemia, the hallmark of diabetes mellitus, is considered one of the endothelial dysfunction risk factors, the main reason of vascular complication. In this study, we aimed to evaluate homocysteine (Hcy) and asymmetrical dimethylarginine (ADMA) levels in diabetic rats and the possibility to attenuate the elevation of these two parameters by supplementation of docosahexaenoic acid (DHA) alone or loaded zinc oxide nanoparticles (ZnONPs) to improve endothelial dysfunction in streptozotocin (STZ)-induced diabetic rats. Forty male albino rats weighing 180-200 g were classified as control, diabetic, diabetic treated with DHA, and diabetic treated with DHA-loaded zinc oxide nanoparticles (DHA/ZnONPs) groups. Fasting blood glucose, insulin, ADMA, Hcy, and nitric oxide (NO) were estimated. Fatty acids (linoleic acid (LA), arachidonic acid (AA), DHA, α-linolenic acid (ALA), and oleic acid (OA)) were also evaluated by reversed phase HPLC using a UV detector. The results showed that fasting blood sugar, insulin resistance, LA, AA, OA, ADMA, and Hcy increased significantly in diabetic rats compared with control while fasting insulin, DHA, ALA, and NO decreased significantly in diabetic rats. In both treated groups, fasting blood sugar, insulin resistance, LA, AA, OA, ADMA, and Hcy significantly decreased as compared with the diabetic group while fasting insulin, DHA, ALA, and NO were significantly increased. In conclusion, DHA and DHA/ZnONP supplementation protect against diabetic complications and improve endothelial dysfunction as well as hyperhomocysteinemia in diabetes. DHA/ZnONP-treated group appeared more efficient than DHA alone.
高血糖是糖尿病的标志，被认为是内皮功能障碍的危险因素之一，也是血管并发症的主要原因。在这项研究中，我们旨在评估糖尿病大鼠中亚精胺（Hcy）和不对称二甲基精氨酸（ADMA）的水平，以及单独补充二十二碳六烯酸（DHA）或负载氧化锌纳米粒子（ZnONPs）是否有可能减轻这两种参数的升高，从而改善链脲佐菌素（STZ）诱导的糖尿病大鼠的内皮功能障碍。40 只雄性白化大鼠，体重 180-200g，分为对照组、糖尿病组、糖尿病用 DHA 治疗组和糖尿病用负载氧化锌纳米粒子的 DHA 治疗组（DHA/ZnONPs 组）。检测空腹血糖、胰岛素、ADMA、Hcy 和一氧化氮（NO）。还通过反相高效液相色谱法（HPLC）并用紫外检测器评估脂肪酸（亚油酸（LA）、花生四烯酸（AA）、二十二碳六烯酸（DHA）、α-亚麻酸（ALA）和油酸（OA））。结果显示，与对照组相比，糖尿病大鼠的空腹血糖、胰岛素抵抗、LA、AA、OA、ADMA 和 Hcy 显著升高，而空腹胰岛素、DHA、ALA 和 NO 显著降低。在两个治疗组中，与糖尿病组相比，空腹血糖、胰岛素抵抗、LA、AA、OA、ADMA 和 Hcy 显著降低，而空腹胰岛素、DHA、ALA 和 NO 显著增加。综上所述，DHA 和 DHA/ZnONP 补充剂可预防糖尿病并发症，并改善糖尿病患者的内皮功能障碍和高同型半胱氨酸血症。DHA/ZnONP 治疗组的效果优于单独使用 DHA。
