体外葡萄糖对人红细胞中乙二醛酶系统的修饰作用。
Modification of the glyoxalase system in human red blood cells by glucose in vitro.
作者信息
Thornalley P J
机构信息
MRC Mechanisms of Drug Toxicity Group, Aston University, Birmingham, U.K.
出版信息
Biochem J. 1988 Sep 15;254(3):751-5. doi: 10.1042/bj2540751.
The human red-blood-cell glyoxalase system was modified by incubation with high concentrations of glucose in vitro. Red-blood-cell suspensions (50%, v/v) were incubated with 5 mM- and 25 mM-glucose to model normal and hyperglycaemic glucose metabolism. There was an increase in the flux of methylglyoxal metabolized to D-lactic acid via the glyoxalase pathway with high glucose concentration. The increase was approximately proportional to initial glucose concentration over the range studied (5-100 mM). The activities of glyoxalase I and glyoxalase II were not significantly changed, but the concentrations of the glyoxalase substrates, methylglyoxal and S-D-lactoylglutathione, and the percentage of glucotriose metabolized via the glyoxalase pathway, were significantly increased. The increase in the flux of intermediates metabolized via the glyoxalase pathway during periodic hyperglycaemia may be a biochemical factor involved in the development of chronic clinical complications associated with diabetes mellitus.
通过在体外与高浓度葡萄糖孵育，对人红细胞乙二醛酶系统进行了修饰。将红细胞悬液（50%，v/v）与5 mM和25 mM葡萄糖孵育，以模拟正常和高血糖状态下的葡萄糖代谢。在高葡萄糖浓度下，通过乙二醛酶途径代谢为D-乳酸的甲基乙二醛通量增加。在所研究的范围内（5-100 mM），这种增加与初始葡萄糖浓度大致成比例。乙二醛酶I和乙二醛酶II的活性没有显著变化，但乙二醛酶底物甲基乙二醛和S-D-乳酰谷胱甘肽以及通过乙二醛酶途径代谢的葡萄糖三糖的百分比显著增加。周期性高血糖期间通过乙二醛酶途径代谢的中间产物通量增加可能是与糖尿病相关的慢性临床并发症发生发展中的一个生化因素。
Zotero 插件