Olshinka Asaf, Ad-El Dean, Didkovski Elena, Weiss Shirel, Ankri Rinat, Goldenberg-Cohen Nitza, Fixler Dror
Department of Plastic Surgery, Rabin Medical Center-Beilinson Hospital, Petach Tikva 4941492, Israel.
Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 6997801, Israel.
Materials (Basel). 2020 Jan 17;13(2):447. doi: 10.3390/ma13020447.
Diffusion reflectance spectroscopy measurements targeted with gold nanoparticles (GNPs) can identify residual cutaneous squamous cell carcinoma (SCC) in excision borders. Human SCC specimens were stained with hematoxylin and eosin to identify tumor borders, and reflected onto an unstained deparaffinized section. Diffusion reflection of three sites (normal and SCC) were measured before and after GNPs targeting. Hyperspectral imaging showed a mean of 2.5 sites with tumor per specimen and 1.2 tumor-free ( < 0.05, -test). GNPs were detected in 25/30 tumor sites (sensitivity 83.3%, false-negative rate 16.6%) and 12/30 non-tumor sites (specificity 60%, false-positive rate 40%). This study verifies the use of nanotechnology in identifying SCC tumor margins. Diffusion reflection scanning has high sensitivity for detecting the residual tumor.
以金纳米颗粒（GNP）为靶向的扩散反射光谱测量可识别切除边缘残留的皮肤鳞状细胞癌（SCC）。将人类SCC标本用苏木精和伊红染色以确定肿瘤边界，并将其反射到未染色的脱石蜡切片上。在GNP靶向前后测量三个部位（正常和SCC）的扩散反射。高光谱成像显示每个标本平均有2.5个肿瘤部位和1.2个无肿瘤部位（<0.05，检验）。在25/30个肿瘤部位检测到GNP（敏感性83.3%，假阴性率16.6%），在12/30个非肿瘤部位检测到GNP（特异性60%，假阳性率40%）。本研究验证了纳米技术在识别SCC肿瘤边缘中的应用。扩散反射扫描对检测残留肿瘤具有高敏感性。
