Misaka Beatrice C, Wosula Everlyne N, Marchelo-d'Ragga Philip W, Hvoslef-Eide Trine, Legg James P
Department of Agricultural Science, School of Natural Resources and Environmental Sciences, University of Juba, P.O. Box 82, Juba, South Sudan.
Department of Plant Sciences, Norwegian University of Life Sciences (NMBU), P.O. Box 5003, 1432 Ås, Norway.
Insects. 2020 Jan 17;11(1):58. doi: 10.3390/insects11010058.
(Gennadius) is a polyphagous, highly destructive pest that is capable of vectoring viruses in most agricultural crops. Currently, information regarding the distribution and genetic diversity of in South Sudan is not available. The objectives of this study were to investigate the genetic variability of infesting sweet potato and cassava in South Sudan. Field surveys were conducted between August 2017 and July and August 2018 in 10 locations in Juba County, Central Equatoria State, South Sudan. The sequences of mitochondrial DNA cytochrome oxidase I (mtCOI) were used to determine the phylogenetic relationships between sampled . Six distinct genetic groups of were identified, including three non-cassava haplotypes (Mediterranean (MED), Indian Ocean (IO), and Uganda) and three cassava haplotypes (Sub-Saharan Africa 1 sub-group 1 (SSA1-SG1), SSA1-SG3, and SSA2). MED predominated on sweet potato and SSA2 on cassava in all of the sampled locations. The Uganda haplotype was also widespread, occurring in five of the sampled locations. This study provides important information on the diversity of species in South Sudan. A comprehensive assessment of the genetic diversity, geographical distribution, population dynamics, and host range of species in South Sudan is vital for its effective management.
（根纳迪乌斯）是一种多食性、极具破坏性的害虫，能够在大多数农作物中传播病毒。目前，关于南苏丹该害虫的分布和遗传多样性的信息尚不可知。本研究的目的是调查南苏丹侵害甘薯和木薯的该害虫的遗传变异性。2017年8月至2018年7月及8月期间，在南苏丹中赤道州朱巴县的10个地点进行了实地调查。利用线粒体DNA细胞色素氧化酶I（mtCOI）序列来确定所采集的该害虫之间的系统发育关系。鉴定出了该害虫的六个不同遗传组，包括三个非木薯单倍型（地中海型（MED）、印度洋型（IO）和乌干达型）以及三个木薯单倍型（撒哈拉以南非洲1亚组1（SSA1-SG1）、SSA1-SG3和SSA2）。在所有采样地点，MED在甘薯上占主导，SSA2在木薯上占主导。乌干达单倍型也广泛分布，在五个采样地点出现。本研究提供了关于南苏丹该害虫物种多样性的重要信息。对南苏丹该害虫物种的遗传多样性、地理分布、种群动态和寄主范围进行全面评估对其有效管理至关重要。
