National Crops Resources Research Institute P.O. Box 7084, Kampala, Uganda ; School of Molecular and Cell Biology, University of the Witwatersrand P.O. Box 2050, BraamFontein, Johannesburg, South Africa.
Ecol Evol. 2012 Nov;2(11):2749-62. doi: 10.1002/ece3.379. Epub 2012 Oct 1.
The genetic variability of whitefly (Bemisia tabaci) species, the vectors of cassava mosaic begomoviruses (CMBs) in cassava growing areas of Kenya, Tanzania, and Uganda, was investigated through comparison of partial sequences of the mitochondria cytochrome oxidase I (mtCOI) DNA in 2010/11. Two distinct species were obtained including sub-Saharan Africa 1 (SSA1), comprising of two sub-clades (I and II), and a South West Indian Ocean Islands (SWIO) species. Among the SSA1, sub-clade I sequences shared a similarity of 97.8-99.7% with the published Uganda 1 genotypes, and diverged by 0.3-2.2%. A pairwise comparison of SSA1 sub-clade II sequences revealed a similarity of 97.2-99.5% with reference southern Africa genotypes, and diverged by 0.5-2.8%. The SSA1 sub-clade I whiteflies were widely distributed in East Africa (EA). In comparison, the SSA1 sub-clade II whiteflies were detected for the first time in the EA region, and occurred predominantly in the coast regions of Kenya, southern and coast Tanzania. They occurred in low abundance in the Lake Victoria Basin of Tanzania and were widespread in all four regions in Uganda. The SWIO species had a sequence similarity of 97.2-97.7% with the published Reunion sequence and diverged by 2.3-2.8%. The SWIO whiteflies occurred in coast Kenya only. The sub-Saharan Africa 2 whitefly species (Ug2) that was associated with the severe CMD pandemic in Uganda was not detected in our study.
2010/11 年，通过比较线粒体细胞色素氧化酶 I（mtCOI）DNA 的部分序列，研究了肯尼亚、坦桑尼亚和乌干达木薯种植区烟粉虱（Bemisia tabaci）物种，即木薯镶嵌病毒（CMBs）的载体的遗传变异性。获得了两个不同的物种，包括撒哈拉以南非洲 1 型（SSA1），包括两个亚群（I 和 II）和西南印度洋岛屿（SWIO）物种。在 SSA1 中，亚群 I 序列与已发表的乌干达 1 基因型的相似性为 97.8-99.7%，差异为 0.3-2.2%。SSA1 亚群 II 序列的成对比较显示，与参考的南非基因型的相似性为 97.2-99.5%，差异为 0.5-2.8%。SSA1 亚群 I 粉虱广泛分布于东非（EA）。相比之下，SSA1 亚群 II 粉虱首次在 EA 地区检测到，主要发生在肯尼亚的沿海地区、坦桑尼亚的南部和沿海地区。它们在坦桑尼亚维多利亚湖盆地的数量较少，在乌干达的四个地区广泛分布。SWIO 物种与已发表的留尼汪序列的序列相似性为 97.2-97.7%，差异为 2.3-2.8%。SWIO 粉虱仅发生在肯尼亚沿海地区。与乌干达严重 CMD 大流行相关的撒哈拉以南非洲 2 型粉虱（Ug2）在本研究中未检测到。
