An Feng, Yin Xin, Jueraiti Kaibire, Yang Yuanyuan, Yan Zhuoyang, Li Jie, Shan Dongqian
College of Horticulture, Northwest A & F University, Yangling 712100, China.
Plants (Basel). 2025 Apr 12;14(8):1201. doi: 10.3390/plants14081201.
The NAC (NAM, ATAF1/2, and CUC2) family is one of the largest plant-specific transcription factor families, playing a crucial role in adaptation to abiotic stresses. However, the gene family in sweet cherry ( L.) remains poorly understood. In this study, we identified 130 genes () from the sweet cherry genome, which were unevenly distributed across eight chromosomes. Phylogenetic analysis classified the PaNACs into 21 distinct groups, including 2 sweet cherry-specific groups. Comparative analysis revealed significant variations in gene proportions, exon-intron structures, and motif compositions among different groups. Furthermore, cis-element analysis suggested the potential roles of in regulating plant growth, development, hormone signaling, and stress responses. Transcriptomic data revealed tissue-specific expression patterns for several genes. qRT-PCR further confirmed that eight selected were responsive to various abiotic stresses in Gisela 6, a widely used hybrid rootstock in sweet cherry production that shares high sequence similarity in genes with . These findings provide valuable insights for future research on the functional characteristics of the genes in the growth, development, and responses to abiotic stress in sweet cherry.
NAC（NAM、ATAF1/2和CUC2）家族是最大的植物特异性转录因子家族之一，在适应非生物胁迫中起着关键作用。然而，甜樱桃（Prunus avium L.）中的该基因家族仍了解甚少。在本研究中，我们从甜樱桃基因组中鉴定出130个PaNAC基因，它们不均匀地分布在8条染色体上。系统发育分析将PaNACs分为21个不同的组，包括2个甜樱桃特有的组。比较分析揭示了不同组之间基因比例、外显子-内含子结构和基序组成的显著差异。此外，顺式作用元件分析表明PaNACs在调节植物生长、发育、激素信号传导和胁迫反应中的潜在作用。转录组数据揭示了几个PaNAC基因的组织特异性表达模式。qRT-PCR进一步证实，在甜樱桃生产中广泛使用的杂交砧木吉塞拉6号中，8个选定的PaNAC基因对各种非生物胁迫有响应，该砧木在PaNAC基因上与甜樱桃具有高度序列相似性。这些发现为未来研究甜樱桃中PaNAC基因在生长、发育和对非生物胁迫反应中的功能特性提供了有价值的见解。
