Sachan Richa, Kundu Amit, Dey Prasanta, Son Ji Yeon, Kim Kyeong Seok, Lee Da Eun, Kim Hae Ri, Park Jae Hyeon, Lee Su Hyun, Kim Jung-Hwan, Cao Shugeng, Lee Byung Mu, Kwak Jong Hwan, Kim Hyung Sik
School of Pharmacy, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon 16419, Korea.
Department of Pharmacology, College of Medicine, Institute of Health Sciences, Gyeongsang National University, Jinju 52727, Korea.
Antioxidants (Basel). 2020 Jan 19;9(1):84. doi: 10.3390/antiox9010084.
The aquatic extract of (DP) is typically consumed as a beverage in Korea and China and is also used in various traditional medicines. However, the functional role of DP on diabetes-induced renal fibrosis is unclear. Here, the protective effects of DP extract against diabetes-induced renal fibrosis were evaluated. Streptozotocin (STZ, 60 mg/kg) was injected intraperitoneally in rats to induce diabetes. After 5 days, DP extract (25 mg/kg/day) and metformin (50 mg/kg/day) were administered orally to diabetic rats for 28 days. DP administration protected both body and organ weight loss in STZ-treated diabetic rats. Significant improvements in serum blood urea nitrogen (BUN), creatinine, and oxidative stress parameters were observed in diabetic rats by DP administration. DP extract markedly protected diabetic-induced histopathological damages in the kidney and pancreas. A significant reduction was observed in microalbumin, kidney injury molecule-1 (KIM-1), selenium binding protein-1 (SBP1), and pyruvate kinase muscle isozyme M2 (PKM2) levels in the urinary excretion of diabetic rats after the administration of DP extract. The expression of pro-inflammatory cytokines and fibrosis marker levels were significantly reduced in the kidney of diabetic rats. Our results strongly indicate that DP extract exhibits protective activity against diabetes-induced renal fibrosis through ameliorating oxidative stress and inflammation. Therefore, we suggest that DP extract can be used as a preventive agent on the progression of diabetic nephropathy and renal fibrosis.
（某提取物，文中未明确写出具体名称，暂用DP指代）的水提取物在韩国和中国通常作为一种饮品饮用，并且也用于各种传统药物中。然而，DP对糖尿病诱导的肾纤维化的功能作用尚不清楚。在此，评估了DP提取物对糖尿病诱导的肾纤维化的保护作用。将链脲佐菌素（STZ，60mg/kg）腹腔注射到大鼠体内以诱导糖尿病。5天后，将DP提取物（25mg/kg/天）和二甲双胍（50mg/kg/天）口服给予糖尿病大鼠，持续28天。给予DP可保护经STZ处理的糖尿病大鼠的体重和器官重量减轻。给予DP后，糖尿病大鼠的血清血尿素氮（BUN）、肌酐和氧化应激参数有显著改善。DP提取物显著保护了糖尿病诱导的肾脏和胰腺组织病理学损伤。给予DP提取物后，糖尿病大鼠尿排泄中的微量白蛋白、肾损伤分子-1（KIM-1）、硒结合蛋白-1（SBP1）和丙酮酸激酶肌肉同工酶M2（PKM2）水平显著降低。糖尿病大鼠肾脏中促炎细胞因子的表达和纤维化标志物水平显著降低。我们的结果强烈表明，DP提取物通过改善氧化应激和炎症对糖尿病诱导的肾纤维化具有保护活性。因此，我们建议DP提取物可作为预防糖尿病肾病和肾纤维化进展的药物。
