Krauss D, Spahn H, Mutschler E
Department of Pharmacology, University of Frankfurt/Main, Fed. Rep. of Germany.
Arzneimittelforschung. 1988 Oct;38(10):1533-6.
An assay method for the quantification of the centrally acting muscle relaxant baclofen in human plasma and urine is described. Baclofen is separated from biological samples using Sep-Pak C18-cartridges. The liquid-solid extraction is followed by ion-pair extraction. The following procedure involves the formation of the baclofen butyl ester and an additional ion-pair extraction of the ester. Then a fluorescent derivatisation is performed, using the fluorescence marker benoxaprofen chloride. The resulting amide is separated from interfering compounds by TLC (silica gel) and quantified by directly measuring the fluorescence (313 nm/365 nm). The procedure described can also be applied for the determination of the fluoro analogue of baclofen. The lower limit of detection is 10 ng per 1 ml plasma and 20 ng per 0.1 ml urine. The applicability of the method was proved by investigating plasma and urine samples of 2 volunteers after oral administration of 20 mg baclofen as single dose.
本文描述了一种定量测定人血浆和尿液中中枢性肌肉松弛剂巴氯芬的分析方法。使用Sep - Pak C18柱从生物样品中分离巴氯芬。液 - 固萃取后进行离子对萃取。后续步骤包括形成巴氯芬丁酯以及对该酯进行额外的离子对萃取。然后，使用荧光标记物氯贝诺酯进行荧光衍生化。通过TLC（硅胶）将所得酰胺与干扰化合物分离，并通过直接测量荧光（313nm/365nm）进行定量。所描述的方法也可用于测定巴氯芬的氟类似物。检测下限为每1ml血浆10ng和每0.1ml尿液20ng。通过对2名志愿者单次口服20mg巴氯芬后的血浆和尿液样本进行研究，证明了该方法的适用性。
