薄层色谱或高效液相色谱分离后荧光法测定人血浆中的左丙氧芬。
Fluorimetric determination of levoprotiline in human plasma after thin-layer chromatographic or high performance liquid chromatographic separation.
作者信息
Horne C, Spahn H, Mutschler E
机构信息
Pharmakologisches Institut für Naturwissenschaftler, Johann Wolfgang Goethe-Universität, Frankfurt/Main, Fed. Rep. of Germany.
出版信息
Arzneimittelforschung. 1987 Oct;37(10):1179-81.
The quantitative determination of the new antidepressant drug levoprotiline, the R-(-)-enantiomer of oxa-protiline (a-[methylamino)methyl)-9,10-ethanoanthracene-9(10H)-ethanol), in human biological material is described. Analysis is performed by alkaline extraction with n-heptane-isopropanol, subsequent fluorescence derivatisation with NBD chloride (4-chloro-7-nitrobenzofurazan), thin layer or high performance liquid chromatography, and fluorimetric measurement of the derivatisation product (lambda max ex. = 470 nm, lambda max em. = 525 nm). The sensitivity of the procedure (detection limit less than 1 ng/ml) permits the performance of pharmacokinetic studies after therapeutic doses.
本文描述了新型抗抑郁药物左普替林（奥沙普替林的R-(-)-对映体，即α-[(甲氨基)甲基]-9,10-亚乙基蒽-9(10H)-乙醇）在人体生物材料中的定量测定方法。分析过程包括用正庚烷-异丙醇进行碱性萃取，随后用NBD氯化物（4-氯-7-硝基苯并呋咱）进行荧光衍生化，再通过薄层色谱法或高效液相色谱法进行分析，并对衍生化产物进行荧光测定（激发波长最大值 = 470 nm，发射波长最大值 = 525 nm）。该方法的灵敏度（检测限小于1 ng/ml）使得在治疗剂量后能够进行药代动力学研究。
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