Fluorimetric determination of dimethylaminohydroxypropoxytriamterene in biological fluids by direct evaluation of thin-layer chromatograms.

A sensitive and specific thin-layer chromatographic method for the determination of the new antikaliuretic compound dimethylaminohydroxypropoxytriamterene (RPH 2823) in plasma and urine of rats is described. After dilution of the samples with methanol the tlc-separation is performed on silica gel plates in chloroform-methanol and NH3-atmosphere. RPH 2823 is recovered quantitatively from plasma and urine. The limit of detection is 5 ng/ml plasma and 50 ng/ml urine. The standard deviation of the method is 5% or less.