通过直接评估薄层色谱图荧光法测定生物流体中的二甲氨基羟基丙氧基氨苯蝶啶
Fluorimetric determination of dimethylaminohydroxypropoxytriamterene in biological fluids by direct evaluation of thin-layer chromatograms.
作者信息
Kraft H, Mutschler E
出版信息
Arzneimittelforschung. 1985;35(10):1542-4.
PMID:4074411
Abstract
A sensitive and specific thin-layer chromatographic method for the determination of the new antikaliuretic compound dimethylaminohydroxypropoxytriamterene (RPH 2823) in plasma and urine of rats is described. After dilution of the samples with methanol the tlc-separation is performed on silica gel plates in chloroform-methanol and NH3-atmosphere. RPH 2823 is recovered quantitatively from plasma and urine. The limit of detection is 5 ng/ml plasma and 50 ng/ml urine. The standard deviation of the method is 5% or less.
摘要
本文描述了一种灵敏且特异的薄层色谱法，用于测定大鼠血浆和尿液中新型抗利尿化合物二甲氨基羟基丙氧基氨苯蝶啶（RPH 2823）。用甲醇稀释样品后，在硅胶板上于氯仿 - 甲醇和氨气氛围中进行薄层色谱分离。RPH 2823可从血浆和尿液中定量回收。检测限为血浆5 ng/ml和尿液50 ng/ml。该方法的标准偏差为5%或更低。
相似文献
1
2
3
Fluorimetric determination of norfloxacin in plasma and urine samples after thin-layer chromatographic separation.薄层色谱分离后血浆和尿液样本中诺氟沙星的荧光测定法。
Arzneimittelforschung. 1989 Jun;39(6):656-8.
4
Pharmacodynamics and pharmacokinetics of the basic triamterene analogue dimethylaminohydroxypropoxytriamterene.基本氨苯蝶啶类似物二甲基氨基羟基丙氧基氨苯蝶啶的药效学和药代动力学。
Arzneimittelforschung. 1985;35(11):1688-91.
5
Thin-layer and gas chromatographic determination of ketamine and its biotransformed products in biological fluids.生物流体中氯胺酮及其生物转化产物的薄层色谱和气相色谱测定法
Res Commun Chem Pathol Pharmacol. 1976 Jun;14(2):367-76.
6
[On the absorption and excretion of raubasin in rats with particular reference to a method for the direct determination of the alkaloid].[关于萝芙木碱在大鼠体内的吸收与排泄，特别提及一种直接测定该生物碱的方法]
Arzneimittelforschung. 1975 Jun;25(6):870-3.
7
Fluorimetric determination of levoprotiline in human plasma after thin-layer chromatographic or high performance liquid chromatographic separation.薄层色谱或高效液相色谱分离后荧光法测定人血浆中的左丙氧芬。
Arzneimittelforschung. 1987 Oct;37(10):1179-81.
8
9
A new type of two-dimensional thin-layer chromatography (TLC mapping) for analysis of acidic glycosphingolipid molecular species.一种用于分析酸性糖鞘脂分子种类的新型二维薄层色谱法（TLC 图谱法）。
Anal Biochem. 1995 May 1;227(1):195-200. doi: 10.1006/abio.1995.1270.
Zotero 插件