Arizono N, Nakao S
Department of Pathology, Shiga University of Medical Science, Ohtsu, Japan.
APMIS. 1988 Nov;96(11):964-70. doi: 10.1111/j.1699-0463.1988.tb00969.x.
Mucosal mast-cell hyperplasia occurs in the rat small intestine mucosa after infection with Nippostrongylus brasiliensis. In the present study, the number of mast cells was found to increase in the muscularis and subserosa as well as in the mucosa of rat small intestines 2-3 weeks after infection with this nematode. Mast cells in the muscularis were stained blue by the alcian blue/safranin sequence and did not bind berberine sulfate. The staining was blocked when tissues were fixed in neutral formalin. The increase in mast cells was transient and gradually disappeared; the half-life was 40 days. After an intravenous administration of compound 48/80, mast cells in the muscularis did not discharge granules. The results indicate that these mast cells were of the mucosal type. The mast cell phenotype in the muscularis did not change even 12 weeks after infection. Mast cells in the subserosal tissue were first of the mucosal type as were those in muscularis. After 8-12 weeks, however, many subserosal mast cells became positive for berberine sulfate and safranin. These results show that mucosal-type mast cells do not undergo phenotypic changes during the period of observation when these cells appear in the muscularis but the phenotypic expression may change as the cells arise in subserosal tissue.
感染巴西日圆线虫后，大鼠小肠黏膜会出现黏膜肥大细胞增生。在本研究中，发现感染这种线虫2 - 3周后，大鼠小肠的肌层、浆膜下层以及黏膜中的肥大细胞数量增加。肌层中的肥大细胞经阿尔辛蓝/番红染色序列染成蓝色，不与硫酸小檗碱结合。当组织用中性福尔马林固定时，染色被阻断。肥大细胞数量的增加是短暂的，随后逐渐消失；半衰期为40天。静脉注射化合物48/80后，肌层中的肥大细胞不释放颗粒。结果表明这些肥大细胞属于黏膜型。即使在感染12周后，肌层中的肥大细胞表型也没有改变。浆膜下层组织中的肥大细胞最初与肌层中的一样，属于黏膜型。然而，8 - 12周后，许多浆膜下层的肥大细胞对硫酸小檗碱和番红呈阳性。这些结果表明，当黏膜型肥大细胞出现在肌层时，在观察期内其表型不会发生变化，但当这些细胞出现在浆膜下层组织时，其表型表达可能会改变。
