Ziotti Isabella Rodrigues, Paschoini Vitória Leite, Corona Silmara Aparecida Milori, Souza-Gabriel Aline Evangelista
Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Restor Dent Endod. 2022 Jun 15;47(3):e28. doi: 10.5395/rde.2022.47.e28. eCollection 2022 Aug.
Metalloproteinase-inhibiting agents, such as chitosan, can prevent collagen degradation in demineralized dental substrates, thereby improving the adhesive interface. This study evaluated the bond strength (BS) and chemical and morphological characterization of the adhesive interface after applying chitosan solution to demineralized dentin.
The 80 third molars were selected. Forty teeth underwent caries induction using the pH cycling method. The teeth were divided according to the treatment: distilled water (control) and 2.5% chitosan solution. The surfaces were restored using adhesive and composite resins. Half of the specimens in each group were aged, and the other half underwent immediate analyses. The teeth were sectioned and underwent the microtensile bond strength test (µTBS), and chemical and morphological analyses using energy-dispersive spectroscopy and scanning electron microscopy, respectively. Data analysis was performed using 3-way analysis of variance.
For µTBS, sound dentin was superior to demineralized dentin ( < 0.001), chitosan-treated specimens had higher bond strength than the untreated ones ( < 0.001), and those that underwent immediate analysis had higher values than the aged specimens ( = 0.019). No significant differences were observed in the chemical or morphological compositions.
Chitosan treatment improved bond strength both immediately and after aging, even in demineralized dentin.
壳聚糖等金属蛋白酶抑制剂可防止脱矿牙基质中的胶原蛋白降解，从而改善黏附界面。本研究评估了将壳聚糖溶液应用于脱矿牙本质后黏附界面的粘结强度（BS）以及化学和形态特征。
选取80颗第三磨牙。40颗牙齿采用pH循环法诱导龋病。根据处理方式将牙齿分为：蒸馏水（对照组）和2.5%壳聚糖溶液组。使用粘结剂和复合树脂修复表面。每组一半的标本进行老化处理，另一半进行即时分析。将牙齿切片并分别进行微拉伸粘结强度测试（µTBS）以及使用能谱分析和扫描电子显微镜进行化学和形态分析。使用三因素方差分析进行数据分析。
对于µTBS，健康牙本质优于脱矿牙本质（<0.001），壳聚糖处理的标本粘结强度高于未处理的标本（<0.001），即时分析的标本值高于老化标本（=0.019）。在化学或形态组成上未观察到显著差异。
壳聚糖处理即使在脱矿牙本质中，也能即时和老化后均提高粘结强度。
