Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Key Laboratory of Opto Electronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, 350007, P. R. China.
Angew Chem Int Ed Engl. 2020 Apr 6;59(15):5972-5978. doi: 10.1002/anie.201913970. Epub 2020 Feb 19.
The use of surface-enhanced Raman spectroscopy (SERS) to determine spectral markers for the diagnosis of heparin-induced thrombocytopenia (HIT), a difficult-to-diagnose immune-related complication that often leads to limb ischemia and thromboembolism, is proposed. The ability to produce distinct molecular signatures without the addition of labels enables unbiased inquiry and makes SERS an attractive complementary diagnostic tool. A capillary-tube-derived SERS platform offers ultrasensitive, label-free measurement as well as efficient handling of blood serum samples. This shows excellent reproducibility, long-term stability and provides an alternative diagnostic rubric for the determination of HIT by leveraging machine-learning-based classification of the spectroscopic data. We envision that a portable Raman instrument could be combined with the capillary-tube-based SERS analytical tool for diagnosis of HIT in the clinical laboratory, without perturbing the existing diagnostic workflow.
提出了利用表面增强拉曼光谱（SERS）来确定肝素诱导的血小板减少症（HIT）的光谱标志物，HIT 是一种难以诊断的免疫相关并发症，常导致肢体缺血和血栓栓塞。SERS 无需添加标签即可产生独特的分子特征，具有无偏探测的能力，因此成为一种有吸引力的互补诊断工具。基于毛细管的 SERS 平台提供了超灵敏、无标记的测量以及对血清样本的有效处理。该平台显示出极好的重现性、长期稳定性，并通过基于机器学习的光谱数据分析分类为 HIT 的诊断提供了替代的诊断标准。我们设想，便携式拉曼仪器可以与基于毛细管的 SERS 分析工具相结合，用于临床实验室中 HIT 的诊断，而不会干扰现有的诊断工作流程。
