Applied Microbial Processes & Environmental Health Research Group, Department of Microbiology, Faculty of Life Sciences, University of Benin, Benin City, Nigeria.
Department of Environmental Health Sciences, Faculty of Public Health, College of Medicine, University of Ibadan, Ibadan, Nigeria.
J Appl Microbiol. 2020 Jul;129(1):17-36. doi: 10.1111/jam.14590. Epub 2020 Feb 3.
The current study was conducted to determine the incidence, antibiotic resistance and virulence genes of Vibrio strains isolated from ready-to-eat shrimps in Edo and Delta States, Nigeria.
A total of 1440 ready-to-eat shrimp samples were obtained from open markets from November 2016 to October 2017 and analysed using standard culture-based procedures. Overall, our result showed that the ready-to-eat shrimp samples had high mean aerobic mesophilic bacterial count between 3·543 and 7·489 log CFU per gram. Vibrio cell densities ranged between 0·663 and 6·761 log CFU per gram. From the total samples, 1343/1440 (93·3%) were positive for Vibrio species where 120 Vibrio isolates were randomly selected and confirmed using genus- and species-specific PCR approach. The PCR identification revealed the presence of Vibrio parahaemolyticus 46 (38·33%), Vibrio vulnificus 14 (11·67%), Vibrio fluvialis 12 (10%), Vibrio alginolyticus 8 (6·67%), Vibrio cholerae 2 (1·67%), Vibrio mimicus 10 (8·33%), Vibrio harveyi 3 (2·5%) and other Vibrio sp. 25 (20·83%). All Vibrio isolates were sensitive to colistin and gentamycin with varying percentage of resistance to other antibiotics used in the study. Multiple antibiotic-resistant (MAR) index ranged from 0·08 to 0·83. The tcp, tdh and trl virulence genes were identified in 95 (79·2%), 92 (76·7%) and 95 (79·2%) of the examined isolates respectively. Antibiotic-resistant genes also revealed the presence of class 1 integrase 75 (62·5%), sul2 87 (72·5%), strB 94 (78·3%) and catB3 68 (56·7%).
Our findings revealed that the ready-to-eat shrimps may serve as potential reservoirs and medium in the dissemination of prospective MAR pathogens to the consumers and thus constitute a potential risk to public health.
The findings from this study represent the first comprehensive report of Vibrio isolates from ready-to-eat shrimps in Edo and Delta States, Nigeria. Incessant monitoring of Vibrio strains and their predisposition to antimicrobials is a necessity to guarantee seafood safety and guarantee the best treatment regimen for patients with gastroenteritis.
本研究旨在确定尼日利亚江户州和三角洲州即食虾中分离出的霍乱弧菌的发生率、抗生素耐药性和毒力基因。
2016 年 11 月至 2017 年 10 月，采用标准的基于培养的程序从开放式市场采集了 1440 份即食虾样本。总体而言，我们的结果显示，即食虾样本的好氧嗜温细菌总数平均值较高，介于每克 3.543 至 7.489 对数 CFU 之间。弧菌细胞密度介于每克 0.663 至 6.761 对数 CFU 之间。在总样本中，1343/1440（93.3%）为霍乱弧菌阳性，其中随机选择 120 个霍乱弧菌分离株，并使用属和种特异性 PCR 方法进行确认。PCR 鉴定显示，存在副溶血性弧菌 46 株（38.33%）、溶藻弧菌 14 株（11.67%）、弗氏柠檬酸杆菌 12 株（10%）、Alg 弧菌 8 株（6.67%）、霍乱弧菌 2 株（1.67%）、拟态弧菌 10 株（8.33%）、哈维氏弧菌 3 株（2.5%）和其他弧菌 25 株（20.83%）。所有霍乱弧菌分离株均对黏菌素和庆大霉素敏感，对研究中使用的其他抗生素的耐药性存在差异。多重抗生素耐药（MAR）指数为 0.08 至 0.83。95（79.2%）、92（76.7%）和 95（79.2%）个被检测的分离株分别鉴定出 tcp、tdh 和 trl 毒力基因。抗生素耐药基因还显示存在类 1 整合酶 75（62.5%）、sul2 87（72.5%）、strB 94（78.3%）和 catB3 68（56.7%）。
我们的研究结果表明，即食虾可能是潜在的储存库和传播媒介，将潜在的多药耐药病原体传播给消费者，从而对公共健康构成潜在威胁。
本研究结果代表了尼日利亚江户州和三角洲州即食虾中霍乱弧菌的首次综合报告。不断监测弧菌菌株及其对抗菌药物的易感性是确保海产品安全和为肠胃炎患者提供最佳治疗方案的必要条件。
