Applied Microbial Processes and Environmental Health Research Group, Faculty of Life Sciences, University of Benin, Private Mail Bag 1154, Benin City, 300283, Edo State, Nigeria.
Department of Microbiology, College of Natural and Applied Sciences, Western Delta University, Oghara, Delta State, Nigeria.
Sci Rep. 2023 Mar 14;13(1):4186. doi: 10.1038/s41598-023-31359-4.
This study examined the occurrence of V. parahaemolyticus from ready-to-eat (RTE) food in Delta State, Nigeria. It also characterized antibiotic resistance and virulence gene profile patterns to determine the associated health risk hazard. Food samples total of 380 were collected randomly and assessed for V. parahaemolyticus. V. parahaemolyticus isolates were characterized for their virulence and antibiogram potentials using a phenotypic and polymerase chain reaction (PCR) approach. A total of 42 (11.1%) samples were contaminated with V. parahaemolyticus. In 17/42 (40.5%) of the V. parahaemolyticus-positive samples, the densities were < 10 MPN/g. However, 19/42 (45.2%) and 6/42 (14.3%) of the samples had densities of 10 - 10 and > 10 MPN/g, respectively. A total of 67 V. parahaemolyticus isolates were identified using PCR; 54(80.6%) isolates were multidrug resistant. A total of 22 (32.8%), 39 (58.2%), and 67 (100%) of the V. parahaemolyticus harbored the tdh, trh, and tlh toxin genes, respectively. The T3SS1 gene (vcrD1) was detected in 67 (100%) of the isolates. The T3SS2α genes which were vcrD2, vopB2, and vopT were detected in 21 (31.3%), 11 (16.4%) and 30 (44.8%) of the isolates respectively. Some of the V. parahaemolytics strains harbored the orf8 gene 20 (29.9%), and a combination of orf8 + tdh genes 12 (17.9%), categorized as pandemic strains. The antibiotic resistance genes detected in this study include bla 33 (49.3), tetM 19 (28.4), cmlA 32(47.8) and sul1 14 (20.9). The concentration levels and prevalence of V. parahaemolyticus in RTE foods indicate contamination of ready-to-eat foods, particularly street foods consumed in the Delta State of Nigeria, threatening public health and consumer safety.
本研究检测了尼日利亚三角洲州即食（RTE）食品中副溶血性弧菌的发生情况。还对其抗生素耐药性和毒力基因谱模式进行了特征分析，以确定相关的健康危害风险。共采集 380 份食品样本进行副溶血性弧菌检测。采用表型和聚合酶链反应（PCR）方法对副溶血性弧菌分离株进行毒力和抗生素谱分析。共有 42 份（11.1%）样本受到副溶血性弧菌污染。在 42 份副溶血性弧菌阳性样本中，有 17 份（40.5%）的密度<10 MPN/g。然而，19 份（45.2%）和 6 份（14.3%）样本的密度分别为 10 - 10 和>10 MPN/g。共使用 PCR 鉴定出 67 株副溶血性弧菌；54 株（80.6%）分离株为多药耐药株。22 株（32.8%）、39 株（58.2%）和 67 株（100%）副溶血性弧菌分别携带 tdh、trh 和 tlh 毒素基因。67 株（100%）分离株均检测到 T3SS1 基因（vcrD1）。T3SS2α 基因 vcrD2、vopB2 和 vopT 分别在 21 株（31.3%）、11 株（16.4%）和 30 株（44.8%）分离株中检出。部分副溶血性弧菌菌株携带 orf8 基因 20 株（29.9%）和 orf8+tdh 基因组合 12 株（17.9%），归类为流行株。本研究检测到的抗生素耐药基因包括 bla 33（49.3%）、tetM 19（28.4%）、cmlA 32（47.8%）和 sul1 14（20.9%）。RTE 食品中副溶血性弧菌的浓度水平和流行率表明，即食食品，特别是尼日利亚三角洲州食用的街头食品受到污染，威胁着公共健康和消费者安全。
