Applied Microbial Processes & Environmental Health Research Group, Department of Microbiology, Faculty of Life Sciences, University of Benin, PMB, 1154, Benin City, Nigeria.
Applied Microbial Processes & Environmental Health Research Group, Department of Microbiology, Faculty of Life Sciences, University of Benin, PMB, 1154, Benin City, Nigeria.
Microb Pathog. 2018 Jun;119:93-102. doi: 10.1016/j.micpath.2018.04.015. Epub 2018 Apr 11.
In this study, we evaluated the virulence factor production, biofilm-forming ability and cell surface properties of ready-to-eat shrimps associated vibrios strains. A total of 1440 ready-to-eat (RTE) shrimp samples were purchased from open markets in southern Nigeria, from November 2016 to October 2017. Biofilm formation was carried out using the microtitre plate method. Cell-to-cell adhesion of Vibrio species was assessed via surface hydrophobicity using the bacterial adherence to hydrocarbons (BATH) and salting aggregation technique, autoaggregation and coaggregation assay. The virulence potential of the identified 120 Vibrio strains includes haemolysis 107 (89.17%), lipase 106 (88.33%), protease 108 (90%), gelatinase 111 (92.5%), the presence of surface-layer (S-layer) 109 (90.8%) and DNA degrading activity 107 (89.17%). Biofilm formation at 30 °C tryptone soy broth in dynamic conditions revealed total biofilm producers for the Vibrio species as follows: V. parahaemolyticus (95.65%), V. vulnificus (92.86%), V. fluvialis (91.67%), V. alginolyticus (87.5%), V. cholerae (100%), V. mimicus (90%), V. harveyi (66.7%), and other Vibrio spp. (84%). A total of 50 biofilm producing vibrios using BATH technique include 49 (98%) hydrophilic and 1 (2%) moderately hydrophobic. Using the modified salting aggregation technique, 50 (100%) was characterized as hydrophilic. Autoaggregation index for the 12 biofilms producing Vibrio strains ranged from 11.6 to 41.3%, while the autoaggregation index for the 12 test bacteria ranged from 26.2 to 71.3%. Coaggregation between the 12 test bacteria with the 12 Vibrio strains ranged from 9.3 to 78.5%. However most vibrios in this study were hydrophilic, their hydrophilic potential is important for their capability to autoaggregate and coaggregate. Findings on the specific process by which virulent Vibrio spp. form biofilm and adhere to shrimp surface as attached plankton may assist in monitoring epidemics of the pathogen.
在这项研究中，我们评估了即食虾相关弧菌菌株的毒力因子产生、生物膜形成能力和细胞表面特性。2016 年 11 月至 2017 年 10 月，从尼日利亚南部的开放市场购买了 1440 份即食（RTE）虾样本。通过微量滴定板法进行生物膜形成。通过使用细菌对烃类的附着（BATH）和盐析聚集技术、自动聚集和共聚试验评估了种间 Vibrio 细胞间的粘附，评估了 Vibrio 种的疏水性。鉴定的 120 株弧菌的毒力潜能包括溶血 107（89.17%）、脂肪酶 106（88.33%）、蛋白酶 108（90%）、明胶酶 111（92.5%）、表面层（S 层）109（90.8%）和 DNA 降解活性 107（89.17%）。在动态条件下 30°C 胰蛋白胨大豆肉汤中的生物膜形成显示，Vibrio 种的总生物膜生产者如下：副溶血弧菌（95.65%）、创伤弧菌（92.86%）、弗氏弧菌（91.67%）、 Alg 弧菌（87.5%）、霍乱弧菌（100%）、拟态弧菌（90%）、哈维氏弧菌（66.7%）和其他弧菌属（84%）。使用 BATH 技术的总共 50 种生物膜产生弧菌包括 49 种（98%）亲水和 1 种（2%）中度疏水。使用改良的盐析聚集技术，50 种（100%）被表征为亲水。12 种产生物膜的 Vibrio 菌株的自动聚集指数范围为 11.6%至 41.3%，而 12 种测试细菌的自动聚集指数范围为 26.2%至 71.3%。12 种测试细菌与 12 种 Vibrio 菌株之间的共聚指数范围为 9.3%至 78.5%。然而，本研究中的大多数弧菌是亲水的，它们的亲水潜力对于它们的自动聚集和共聚能力很重要。关于毒力弧菌形成生物膜并附着在虾表面作为附着浮游生物的特定过程的发现可能有助于监测病原体的流行。
