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虾青素治疗改善小鼠骨骼肌强直力和线粒体钙稳态

Improved Tetanic Force and Mitochondrial Calcium Homeostasis by Astaxanthin Treatment in Mouse Skeletal Muscle.

作者信息

Sztretye Mónika, Singlár Zoltán, Szabó László, Angyal Ágnes, Balogh Norbert, Vakilzadeh Faranak, Szentesi Péter, Dienes Beatrix, Csernoch László

机构信息

Department of Physiology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.

Doctoral School of Molecular Medicine, University of Debrecen, 4032 Debrecen, Hungary.

出版信息

Antioxidants (Basel). 2020 Jan 23;9(2):98. doi: 10.3390/antiox9020098.

DOI:10.3390/antiox9020098
PMID:31979219
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7070261/
Abstract

BACKGROUND

Astaxanthin (AX) a marine carotenoid is a powerful natural antioxidant which protects against oxidative stress and improves muscle performance. Retinol and its derivatives were described to affect lipid and energy metabolism. Up to date, the effects of AX and retinol on excitation-contraction coupling (ECC) in skeletal muscle are poorly described.

METHODS

18 C57Bl6 mice were divided into two groups: Control and AX supplemented in rodent chow for 4 weeks (AstaReal A1010). In vivo and in vitro force and intracellular calcium homeostasis was studied. In some experiments acute treatment with retinol was employed.

RESULTS

The voltage activation of calcium transients (V) were investigated in single flexor digitorum brevis isolated fibers under patch clamp and no significant changes were found following AX supplementation. Retinol shifted V towards more positive values and decreased the peak F/F of the calcium transients. The amplitude of tetani in the extensor digitorum longus was significantly higher in AX than in control group. Lastly, the mitochondrial calcium uptake was found to be less prominent in AX.

CONCLUSION

AX supplementation increases in vitro tetanic force without affecting ECC and exerts a protecting effect on the mitochondria. Retinol treatment has an inhibitory effect on ECC in skeletal muscle.

摘要

背景

虾青素（AX）是一种海洋类胡萝卜素，是一种强大的天然抗氧化剂，可抵御氧化应激并改善肌肉性能。视黄醇及其衍生物被认为会影响脂质和能量代谢。迄今为止，AX和视黄醇对骨骼肌兴奋-收缩偶联（ECC）的影响鲜有描述。

方法

将18只C57Bl6小鼠分为两组：对照组和在啮齿动物饲料中添加AX 4周的组（AstaReal A1010）。研究了体内和体外的力量以及细胞内钙稳态。在一些实验中采用了视黄醇急性处理。

结果

在膜片钳下对分离的单一趾短屈肌纤维中的钙瞬变电压激活（V）进行了研究，补充AX后未发现显著变化。视黄醇使V向更正的值移动，并降低了钙瞬变的峰值F/F。AX组趾长伸肌的强直收缩幅度显著高于对照组。最后，发现AX组线粒体钙摄取不那么明显。

结论

补充AX可增加体外强直收缩力，而不影响ECC，并对线粒体发挥保护作用。视黄醇处理对骨骼肌的ECC有抑制作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d391/7070261/b6f0fa5b2e5c/antioxidants-09-00098-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d391/7070261/cee484cdb83e/antioxidants-09-00098-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d391/7070261/e1723eaee53f/antioxidants-09-00098-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d391/7070261/d9f93df8891e/antioxidants-09-00098-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d391/7070261/555d16f5338f/antioxidants-09-00098-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d391/7070261/04eb999db3b8/antioxidants-09-00098-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d391/7070261/b6f0fa5b2e5c/antioxidants-09-00098-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d391/7070261/cee484cdb83e/antioxidants-09-00098-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d391/7070261/e1723eaee53f/antioxidants-09-00098-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d391/7070261/d9f93df8891e/antioxidants-09-00098-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d391/7070261/555d16f5338f/antioxidants-09-00098-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d391/7070261/04eb999db3b8/antioxidants-09-00098-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d391/7070261/b6f0fa5b2e5c/antioxidants-09-00098-g006.jpg

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Oxid Med Cell Longev. 2019 Nov 11;2019:3849692. doi: 10.1155/2019/3849692. eCollection 2019.
2
Effects of astaxanthin supplementation and electrical stimulation on muscle atrophy and decreased oxidative capacity in soleus muscle during hindlimb unloading in rats.虾青素补充和电刺激对大鼠后肢去负荷时比目鱼肌萎缩和氧化能力下降的影响。
J Physiol Sci. 2019 Sep;69(5):757-767. doi: 10.1007/s12576-019-00692-7. Epub 2019 Jul 4.
3
The mitochondrial calcium uniporter underlies metabolic fuel preference in skeletal muscle.
腺苷 A3 受体缺失可加速心肌毒素诱导损伤后小鼠骨骼肌的再生。
Cell Death Dis. 2023 Oct 28;14(10):706. doi: 10.1038/s41419-023-06228-7.
4
Genetic Manipulation of CB1 Cannabinoid Receptors Reveals a Role in Maintaining Proper Skeletal Muscle Morphology and Function in Mice.遗传操纵 CB1 大麻素受体揭示了其在维持小鼠骨骼肌形态和功能中的作用。
Int J Mol Sci. 2022 Dec 9;23(24):15653. doi: 10.3390/ijms232415653.
5
Astaxanthin Exerts Anabolic Effects via Pleiotropic Modulation of the Excitable Tissue.虾青素通过对兴奋组织的多效调节发挥合成代谢作用。
Int J Mol Sci. 2022 Jan 14;23(2):917. doi: 10.3390/ijms23020917.
6
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7
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4
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7
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8
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9
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10
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