Singlár Zoltán, Szentesi Péter, Fodor János, Angyal Ágnes, Csernoch László, Sztretye Mónika
Department of Physiology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.
Doctoral School of Molecular Medicine, University of Debrecen, 4032 Debrecen, Hungary.
Antioxidants (Basel). 2021 Sep 4;10(9):1415. doi: 10.3390/antiox10091415.
Aging and frailty are associated with a decline in muscle force generation, which is a direct consequence of reduced muscle quantity and quality. Among the leading contributors to aging is the generation of reactive oxygen species, the byproducts of terminal oxidation. Their negative effects can be moderated via antioxidant supplementation. Krill oil and astaxanthin (AX) are nutraceuticals with a variety of health promoting, geroprotective, anti-inflammatory, anti-diabetic and anti-fatigue effects. In this work, we examined the functional effects of these two nutraceutical agents supplemented via pelleted chow in aging mice by examining in vivo and in vitro skeletal muscle function, along with aspects of intracellular and mitochondrial calcium homeostasis, as well as cognition and spatial memory. AX diet regimen limited weight gain compared to the control group; however, this phenomenon was not accompanied by muscle tissue mass decline. On the other hand, both AX and krill oil supplementation increased force production without altering calcium homeostasis during excitation-contraction coupling mechanism or mitochondrial calcium uptake processes. We also provide evidence of improved spatial memory and learning ability in aging mice because of krill oil supplementation. Taken together, our data favors the application of antioxidant nutraceuticals as geroprotectors to improve cognition and healthy aging by virtue of improved skeletal muscle force production.
衰老和身体虚弱与肌肉力量生成的下降有关，这是肌肉量和质量降低的直接后果。衰老的主要促成因素之一是活性氧的产生，即末端氧化的副产物。它们的负面影响可通过补充抗氧化剂来缓解。磷虾油和虾青素（AX）是具有多种促进健康、保护衰老、抗炎、抗糖尿病和抗疲劳作用的营养保健品。在这项研究中，我们通过检测体内和体外骨骼肌功能，以及细胞内和线粒体钙稳态方面，以及认知和空间记忆，研究了通过颗粒饲料补充这两种营养保健品对衰老小鼠的功能影响。与对照组相比，AX饮食方案限制了体重增加；然而，这一现象并未伴随着肌肉组织质量的下降。另一方面，补充AX和磷虾油均增加了力量产生，而在兴奋-收缩偶联机制或线粒体钙摄取过程中并未改变钙稳态。我们还提供了证据表明，补充磷虾油可改善衰老小鼠的空间记忆和学习能力。综上所述，我们的数据支持将抗氧化营养保健品作为保护衰老的物质应用，通过改善骨骼肌力量产生来提高认知能力和实现健康衰老。
