Antenucci Fabio, Arak Homa, Gao Jianyang, Allahgadry Toloe, Thøfner Ida, Bojesen Anders Miki
Department of Veterinary and Animal Sciences, University of Copenhagen, DK-1870 Frederiksberg C, Denmark.
Vaccines (Basel). 2020 Jan 23;8(1):40. doi: 10.3390/vaccines8010040.
is a Gram-negative opportunistic avian pathogen representing an emerging threat to poultry meat and egg production worldwide. To date, no vaccine able to effectively prevent the morbidity associated with infections has been developed yet. Our group previously reported that inoculation of different combinations of outer membrane vesicles (OMVs), FlfA and GtxA-N proteins is effective in preventing lesions caused by infections in layer chickens. Here we report the testing of the efficacy as vaccine prototypes of OMVs isolated by hydrostatic filtration, a simple technique that allows the cost-effective isolation of high yields of OMVs. Layer chickens were immunized with OMVs alone or in combination with FlfA and/or GtxA-N proteins. Subsequent challenge with a heterologous strain showed that immunization with OMVs alone could significantly reduce the lesions following a infection. A second study was carried out to characterize the dose-response (0.25, 2.5 and 25 µg) relationship of OMVs as immunogens, showing that 2.5 μg of OMVs represent the optimal dose to elicit protection in the immunized animals after a similar challenge. Additionally, administration of ≥2.5 μg of OMVs induced specific IgY titers and possibly vertical transfer of immunity.
是一种革兰氏阴性机会性禽病原体，对全球禽肉和蛋生产构成新出现的威胁。迄今为止，尚未开发出能够有效预防与感染相关发病率的疫苗。我们小组先前报告说，接种不同组合的外膜囊泡（OMV）、FlfA和GtxA-N蛋白可有效预防蛋鸡感染引起的病变。在此，我们报告了通过静水压过滤分离的OMV作为疫苗原型的功效测试，静水压过滤是一种简单的技术，可经济高效地分离出高产率的OMV。用OMV单独或与FlfA和/或GtxA-N蛋白联合免疫蛋鸡。随后用异源菌株进行攻毒，结果表明单独用OMV免疫可显著减少感染后的病变。进行了第二项研究，以表征OMV作为免疫原的剂量反应（0.25、2.5和25μg）关系，结果表明2.5μg的OMV是在类似攻毒后在免疫动物中引发保护的最佳剂量。此外，给予≥2.5μg的OMV可诱导特异性IgY滴度，并可能实现免疫的垂直传递。
