Unidad de Biotecnología y Prototipos, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, 54074, Tlalnepantla, Estado de México, Mexico.
Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, Ciudad Politécnica de la Innovación Ingeniero Fausto Elio, s/n, 46022, Valencia, Spain.
Arch Virol. 2020 Mar;165(3):781-784. doi: 10.1007/s00705-020-04528-3. Epub 2020 Jan 24.
In this work, we describe the complete sequence and genome organization of a novel tobamovirus detected in a prickly pear plant (Opuntia sp.) by high-throughput sequencing, tentatively named "opuntia virus 2". The full genome of opuntia virus 2 is 6,453 nucleotides in length and contains four open reading frames (ORFs) coding for the two subunits of the RNA polymerase, the movement protein, and the coat protein, respectively. Phylogenetic analysis using the complete nucleotide sequence revealed that the virus belongs to the genus Tobamovirus (family Virgaviridae), showing the highest nucleotide sequence identity (49.8%) with cactus mild mottle virus (CMMoV), being indicating that it belongs in the Cactaceae subgroup of tobamoviruses.
在这项工作中，我们通过高通量测序描述了一种在仙人掌植物（Opuntia sp.）中检测到的新型烟草花叶病毒的完整序列和基因组组织，暂定名为“opuntia virus 2”。opuntia virus 2 的全基因组长度为 6453 个核苷酸，包含四个开放阅读框（ORFs），分别编码 RNA 聚合酶的两个亚基、运动蛋白和外壳蛋白。基于完整核苷酸序列的系统进化分析表明，该病毒属于烟草花叶病毒属（病毒科），与仙人掌轻度斑驳病毒（CMMoV）的核苷酸序列同源性最高（49.8%），表明它属于烟草花叶病毒的仙人掌亚组。
