Grupo Multidisciplinar de Oncología Traslacional, Institut Universitari d´Investigació en Ciències de la Salut (IUNICS), Universitat de les Illes Balears, Palma de Mallorca, E-07122 Illes Balears, Spain; Instituto de Investigación Sanitaria de las Islas Baleares (IdISBa), Hospital Universitario Son Espases, edificio S, Palma de Mallorca, E-07120, Illes Balears, Spain.
Grupo Multidisciplinar de Oncología Traslacional, Institut Universitari d´Investigació en Ciències de la Salut (IUNICS), Universitat de les Illes Balears, Palma de Mallorca, E-07122 Illes Balears, Spain.
Int J Biochem Cell Biol. 2020 Mar;120:105698. doi: 10.1016/j.biocel.2020.105698. Epub 2020 Jan 22.
Antioxidant defences and oxidative stress are related to development, progression and malignancy of colorectal cancer. However, their role in early stages of cancer remains unknown. More and more recent studies have revealed that non-tumour adjacent tissue is not a normal tissue. Thus, our aim was to analyse protein levels of MnSOD (Manganese Superoxide Dismutase), acMnSOD (Acetylated Manganese superoxide Dismutase), SIRT3 (Sirtuin 3), CuZnSOD (Cupper Zinc Superoxide Dismutase), CAT (Catalase), GPx (Glutathione Peroxidase), and GRd (Glutathione Reductase) both in tumour and non-tumour adjacent tissue from colorectal cancer patients by western blot. Non-tumour adjacent tissue seemed to have higher levels of antioxidant enzymes that detoxify hydrogen peroxide compared to tumour tissue. In contrast, tumour tissue had higher levels of MnSOD and acMnSOD. Furthermore, most of the proteins analysed showed significant differences between stage I and II in both non-tumour adjacent and tumour tissue. This could indicate that antioxidant enzymes, especially MnSOD, play a crucial role in early stages of colorectal cancer in both tissues, so they could be analysed as novel biomarkers to improve colorectal cancer diagnosis.
抗氧化防御和氧化应激与结直肠癌的发展、进展和恶性转化有关。然而，它们在癌症早期的作用尚不清楚。越来越多的最新研究表明，非肿瘤相邻组织不是正常组织。因此，我们的目的是通过 Western blot 分析来自结直肠癌患者肿瘤和非肿瘤相邻组织中 MnSOD（锰超氧化物歧化酶）、acMnSOD（乙酰化锰超氧化物歧化酶）、SIRT3（Sirtuin 3）、CuZnSOD（铜锌超氧化物歧化酶）、CAT（过氧化氢酶）、GPx（谷胱甘肽过氧化物酶）和 GRd（谷胱甘肽还原酶）的蛋白水平。与肿瘤组织相比，非肿瘤相邻组织似乎具有更高水平的可解毒过氧化氢的抗氧化酶。相比之下，肿瘤组织具有更高水平的 MnSOD 和 acMnSOD。此外，大多数分析的蛋白质在非肿瘤相邻组织和肿瘤组织的 I 期和 II 期之间均显示出显著差异。这可能表明抗氧化酶，尤其是 MnSOD，在两种组织的结直肠癌早期阶段发挥着关键作用，因此它们可以作为新的生物标志物来提高结直肠癌的诊断。
