Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
J Control Release. 2020 Apr 10;320:283-292. doi: 10.1016/j.jconrel.2020.01.032. Epub 2020 Jan 23.
Image-guided therapy, combined with imaging and therapeutic action, forms an attractive system because it can induce outstanding effects at focused locations. However, the conventional liposomes-based system cannot figure in therapeutic or imaging roles themselves, thereby causing the disadvantage of their biological unavailability as a theragnosis tool. Herein, the structure-inherent near-infrared bilayer nanovesicles are fabricated with amphiphilic heptamethine cyanine dye, PEG conjugated heptamethine cyanine dye, and gemcitabine (NEPCG) is developed for the novel photoacoustic image-guided chemo-thermotherapy system. The organic structure-inherent near-infrared bilayer nanovesicles are self-assembled and exhibit a liposome-like bilayer structure. Furthermore, NEPCG showed the high photoacoustic signal (PA) due to the specific accumulation in the tumor site. Delivered NEPCG than displayed concurrent chemotherapy and photothermal therapy (PTT) effects against cancer, triggered by PA imaging with minimal side effects. In vitro and in vivo experiments show that NEPCG can be used as outstanding contrast agents and completely obliterate the tumor without reoccurrence under laser irradiation. Therefore, this work presents the potential for the realization of unprecedented structure-inherent near-infrared bilayer nanovesicles as highly accurate and effective theragnostic tools in clinical fields.
影像引导治疗结合影像学和治疗作用，形成了一个有吸引力的系统，因为它可以在聚焦部位产生显著的效果。然而，传统的基于脂质体的系统本身不能起到治疗或成像的作用，从而导致其作为治疗工具的生物利用度不足的缺点。在此，通过两亲性七甲川花菁染料、PEG 连接的七甲川花菁染料和吉西他滨（NEPCG）制备了具有内在结构的近红外双层纳米囊泡，用于新型光声图像引导化疗-热疗系统。有机结构内在的近红外双层纳米囊泡自组装形成类似脂质体的双层结构。此外，由于在肿瘤部位的特异性积累，NEPCG 表现出高的光声信号（PA）。与光声成像相关的低副作用触发，递送的 NEPCG 表现出协同化疗和光热治疗（PTT）作用，对抗癌症。体外和体内实验表明，NEPCG 可用作出色的对比剂，在激光照射下完全消除肿瘤且无复发。因此，这项工作为实现前所未有的内在结构近红外双层纳米囊泡作为临床领域高度准确和有效的治疗工具提供了潜力。
