Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan 46241, Republic of Korea.
Department of Laboratory Medicine and Biomedical Research Institute, Pusan National University Hospital and Pusan National University School of Medicine, Busan 49241, Republic of Korea.
Nanotheranostics. 2022 Jan 1;6(1):50-61. doi: 10.7150/ntno.63222. eCollection 2022.
Biomedical imaging is an essential tool for investigating biological responses . Among the several imaging techniques, optical imaging systems with multispectral analysis of nanoparticles have been widely investigated due to their ability to distinguish the substances in biological tissues . This review article focus on multispectral optical imaging techniques that can provide molecular functional information. We summarize the basic principle of the spectral unmixing technique that enables the delineation of optical chromophores. Then, we explore the principle, typical system configuration, and biomedical applications of the representative optical imaging techniques, which are fluorescence imaging, two-photon microscopy, and photoacoustic imaging. The results in the recent studies show the great potential of the multispectral analysis techniques for monitoring responses of biological systems .
生物医学成像是研究生物反应的重要工具。在几种成像技术中，由于能够区分生物组织中的物质，基于纳米粒子的多光谱分析的光学成像系统得到了广泛的研究。本文综述了多光谱光学成像技术，该技术可以提供分子功能信息。我们总结了使光谱解混技术能够描绘光学色团的基本原理。然后，我们探讨了代表性的光学成像技术，即荧光成像、双光子显微镜和光声成像的原理、典型系统结构和生物医学应用。最近的研究结果表明，多光谱分析技术在监测生物系统反应方面具有巨大的潜力。
