Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610065, Sichuan, PR China.
Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610065, Sichuan, PR China.
Microb Pathog. 2020 Apr;141:103998. doi: 10.1016/j.micpath.2020.103998. Epub 2020 Jan 23.
Avian infectious bronchitis virus (IBV) is a coronavirus which infects chickens and causes severe economic losses to the poultry industry worldwide. MicroRNAs (miRNAs) are important intracellular regulators and play a pivotal role in viral infections. In previous studies, we have revealed that IBV infection caused a significant down-regulation of gga-miR-30d expression in chicken kidneys. In present study, we investigated the role of gga-miR-30d in the process of IBV infection of HD11 cell line in vitro. By transfecting the mimics and inhibitor of gga-miR-30d, it was found that overexpressed gga-miR-30d inhibited IBV replication. Contrarily, low-expressed gga-miR-30d promoted IBV replication. In addition, dual-luciferase reporter assays revealed that ubiquitin-specific protease 47 (USP47), a deubiquitinase-encoding gene, was a target for gga-miR-30d. This is the first study demonstrating that miRNAs regulate IBV replication by regulating the deubiquitinating enzyme (DUBs).
禽传染性支气管炎病毒（IBV）是一种冠状病毒，可感染鸡并导致全球家禽业遭受严重的经济损失。microRNAs（miRNAs）是重要的细胞内调节剂，在病毒感染中发挥关键作用。在以前的研究中，我们已经揭示了 IBV 感染导致鸡肾脏中gga-miR-30d 的表达显著下调。在本研究中，我们研究了 gga-miR-30d 在体外 IBV 感染 HD11 细胞系过程中的作用。通过转染 gga-miR-30d 的模拟物和抑制剂，发现过表达 gga-miR-30d 抑制了 IBV 的复制。相反，低表达的 gga-miR-30d 促进了 IBV 的复制。此外，双荧光素酶报告基因实验表明，泛素特异性蛋白酶 47（USP47），一种编码去泛素化酶的基因，是 gga-miR-30d 的靶基因。这是首次研究表明 miRNA 通过调节去泛素化酶（DUBs）来调节 IBV 的复制。
