Dantas Roberto Nery, Souza Augusto Monteiro de, Herrero Sylvia Satomi Takeno, Kassab Paulo, Malheiros Carlos Alberto, Lima Eleonidas Moura
Laboratory of Molecular and Structural Biology Oncogenetics, LBMEO, Federal University of Paraiba; João Pessoa - PB, Brazil.
Postgraduation Program in Health Sciences, Santa Casa de São Paulo Medical Sciences Faculty, Sao Paulo - SP, Brazil.
Asian Pac J Cancer Prev. 2020 Jan 1;21(1):43-48. doi: 10.31557/APJCP.2020.21.1.43.
To evaluate the association of allelic and genotypic frequencies of PSCA (rs2976392), TNF-α (rs1800629), PARP1 (rs1136410) and TP53 (rs368771578) SNPs with GC susceptibility in a Brazilian population.
This is a retrospective study, which included 102 paraffin-embedded adenocarcinoma tissue samples > 5 years of obtention, with 204 alleles for each studied SNP. Other 102 healthy tissue samples were included as controls. For analysis, the genotyping method Dideoxy Single Allele-Specific - PCR was used. Statistical analysis was performed with the Bioestat software 5.3, determining Hardy-Weinberg's equilibrium for the genotypic frequencies p-values < 0.05 were considered significant.
PSCA (rs2976392) and TNF-α (rs1800629) SNPs were associated with GC in the analyzed samples (X2=10.3/102 and p<0.001/0.00001, respectively). TNF-α (rs1800629) SNP presented also a statistically significant relationship between its genotypes and the morphological pattern (intestinal/diffuse) (p<0.032). However, PARP1 (rs1136410) and TP53 (rs368771578) SNPs were in Hardy-Weinberg's equilibrium and, therefore, were not significantly associated with GC in these samples (X2=0.73/2.89 and p<0.39/0.08).
PSCA (rs2976392) and TNF-α (rs1800629) SNPs are potential molecular markers of susceptibility to GC development. PARP1 (rs1136410) and TP53 (rs368771578) SNPs were not associated with the risk of GC development.
评估PSCA（rs2976392）、TNF-α（rs1800629）、PARP1（rs1136410）和TP53（rs368771578）单核苷酸多态性（SNP）的等位基因和基因型频率与巴西人群胃癌易感性之间的关联。
这是一项回顾性研究，纳入了102份获取时间超过5年的石蜡包埋腺癌组织样本，每个研究的SNP有204个等位基因。另外纳入102份健康组织样本作为对照。分析时采用双脱氧单等位基因特异性PCR基因分型方法。使用Bioestat软件5.3进行统计分析，确定基因型频率的哈迪-温伯格平衡，p值<0.05被认为具有统计学意义。
在分析的样本中，PSCA（rs2976392）和TNF-α（rs1800629）SNP与胃癌相关（分别为X2 = 10.3/102和p<0.001/0.00001）。TNF-α（rs1800629）SNP的基因型与形态学类型（肠型/弥漫型）之间也存在统计学显著关系（p<0.032）。然而，PARP1（rs1136410）和TP53（rs368771578）SNP处于哈迪-温伯格平衡，因此在这些样本中与胃癌无显著关联（X2 = 0.73/2.89和p<0.39/0.08）。
PSCA（rs2976392）和TNF-α（rs1800629）SNP是胃癌发生易感性的潜在分子标志物。PARP1（rs1136410）和TP53（rs368771578）SNP与胃癌发生风险无关。
