Ilyas Sajida, Jilani Kashif, Sikandar Muhammad, Siddiq Saba, Riaz Muhammad, Naveed Ayesha, Bibi Ismat, Nawaz Haq, Irfan Muhammad, Asghar Asma
Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan.
Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad, Pakistan.
Dose Response. 2020 Jan 14;18(1):1559325819899259. doi: 10.1177/1559325819899259. eCollection 2020 Jan-Mar.
Naproxen sodium is a nonsteroidal anti-inflammatory drug (NSAID) having antipyretic and analgesic properties, mainly used for the treatment of rheumatoid arthritis and osteoarthritis. Eryptosis is an alternative term used for suicidal erythrocyte death. In the current study, eryptotic effect of naproxen sodium characterized by membrane blebbing was investigated in erythrocytes after 48 hours of treatment with different concentrations (1-25 µM). The experimental work related to investigation of eryptosis was done by cell size measurement and confirmation of calcium role in the induction of membrane blebbing. As a possible mechanism of eryptosis, oxidative stress induced by naproxen sodium was determined by catalase, glutathione peroxidase, and superoxide dismutase activities. Similarly, hemolytic effect of naproxen sodium was also determined by hemolysis measurement. Results of our study illustrated that the therapeutic doses (10-25 µM) of naproxen sodium induce oxidative stress, confirmed by significant decrease in superoxide dismutase, catalase, and glutathione peroxidase activities that lead to the triggering of cell death by eryptosis and hemolysis.
萘普生钠是一种具有解热和镇痛特性的非甾体抗炎药（NSAID），主要用于治疗类风湿性关节炎和骨关节炎。红细胞凋亡是用于描述自杀性红细胞死亡的另一个术语。在本研究中，在用不同浓度（1 - 25 μM）处理48小时后，研究了萘普生钠在红细胞中以膜泡形成为特征的红细胞凋亡效应。与红细胞凋亡研究相关的实验工作通过细胞大小测量以及确认钙在诱导膜泡形成中的作用来完成。作为红细胞凋亡的一种可能机制，通过过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶的活性测定了萘普生钠诱导的氧化应激。同样，萘普生钠的溶血作用也通过溶血测量来确定。我们的研究结果表明，萘普生钠的治疗剂量（10 - 25 μM）会诱导氧化应激，超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性的显著降低证实了这一点，这会导致红细胞凋亡和溶血引发细胞死亡。
