Graduate School of Biotechnology and Department of Oriental Medicinal Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
J Microbiol Biotechnol. 2020 Feb 28;30(2):163-171. doi: 10.4014/jmb.1907.07058.
L. (Solanaceae), commonly known as "angel's trumpet," is widely grown in North America, Africa, Australia, and Asia. It has been mainly used for ornamental purposes as well as analgesic, anti-rheumatic, vulnerary, decongestant, and anti-spasmodic materials. is also reported to show anti-cholinergic activity, for which many alkaloids were reported to be principally responsible. However, to the best of our knowledge, a phytochemical study of flowers has not yet been performed. Four flavonol glycosides () and one dihydroflavanol () were for the first time isolated from flowers in this study. The flavonoids showed significant antioxidant capacities, suppressed nitric oxide production in lipopolysaccharide (LPS)-treated RAW 264.7 cells, and reduced inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) protein production increased by LPS treatment. The contents of compounds in -BuOH fraction were determined to be 3.8 ± 0.9%, 2.2 ± 0.5%, 20.3 ± 1.1%, and 2.3 ± 0.4%, respectively, and that of compound 5 in EtOAc fraction was determined to be 12.7 ± 0.7%, by HPLC experiment. These results suggest that flavonol glycosides () and dihydroflavanol () can serve as index components of flowers in standardizing anti-inflammatory materials.
白花曼陀罗（L.，茄科），俗称“天使的号角”，广泛生长于北美洲、非洲、澳大利亚和亚洲。它主要用于观赏目的，也具有镇痛、抗风湿、生肌、通经和解痉的作用。还报道其具有抗胆碱能活性，许多生物碱主要负责这一作用。然而，据我们所知，尚未对白花曼陀罗花进行植物化学研究。本研究首次从白花曼陀罗花中分离得到四种黄酮醇糖苷（）和一种二氢黄酮醇（）。这些类黄酮表现出显著的抗氧化能力，可抑制脂多糖（LPS）处理的 RAW 264.7 细胞中一氧化氮的产生，并降低 LPS 处理引起的诱导型一氧化氮合酶（iNOS）和环氧化酶（COX-2）蛋白的产生。HPLC 实验测定，-BuOH 部分中化合物的含量分别为 3.8±0.9%、2.2±0.5%、20.3±1.1%和 2.3±0.4%，EtOAc 部分中化合物 5 的含量为 12.7±0.7%。这些结果表明，黄酮醇糖苷（）和二氢黄酮醇（）可作为抗炎材料标准化的白花曼陀罗花的指标成分。
