Nutrition and Biochemistry Department, School of Medicine, Social Development and Health Promotion Research Center, Gonabad University of Medical Sciences, Gonabad, Iran.
Genetic and Molecular Medicine Department, School of Medicine, Mashhad University of Medical Sciences, Iran.
Clin Nutr ESPEN. 2020 Feb;35:103-108. doi: 10.1016/j.clnesp.2019.10.020. Epub 2019 Dec 5.
Animal studies suggest that vitamin D can change the gut microbiome. The primary aim of this study was to evaluate the effect of a high dose supplementation of vitamin D on the composition of the gut microbiome.
After DNA extraction, TaqMan assays were used for the quantitation of selected microbiome in the feces of 50 adolescent girls before and after vitamin D supplementation.
The expression fold changes for Enterococcus, Bifidobacterium, Lactobacillus, Bacteroidetes and Firmicutes were; 1.05, 1.20, 0.76, 0.28 and 1.50 respectively. Bacteroidetes and Lactobacillus fell by 72% (P < 0.0001) and 24% (P = 0.006) respectively, whilst Firmicutes and Bifidobacterium were increased by 1.5 (P < 0.0001), 1.2 (P < 0.0001) fold after supplementation.
Our results suggested that a high dose supplementation of vitamin D alter the human gut microbiome composition. Future studies are required for a better understanding of the mechanisms by which vitamin D affects the gut microbiome.
动物研究表明维生素 D 可以改变肠道微生物组。本研究的主要目的是评估高剂量维生素 D 补充对肠道微生物组组成的影响。
在 DNA 提取后，使用 TaqMan 检测法对 50 名青春期少女在维生素 D 补充前后粪便中的选定微生物组进行定量。
肠球菌、双歧杆菌、乳杆菌、拟杆菌和厚壁菌的表达倍数变化分别为 1.05、1.20、0.76、0.28 和 1.50。拟杆菌和乳杆菌分别下降了 72%（P<0.0001）和 24%（P=0.006），而厚壁菌和双歧杆菌分别增加了 1.5（P<0.0001）和 1.2（P<0.0001）倍。
我们的结果表明，高剂量的维生素 D 补充会改变人类肠道微生物组的组成。需要进一步研究以更好地了解维生素 D 影响肠道微生物组的机制。
