Faculty of Chemical Technology, Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60965 Poznan, Poland.
Institute of Electronics and Sensor Materials, TU Bergakademie Freiberg, Gustav-Zeuner str. 3, 09599 Freiberg, Germany.
Biomolecules. 2020 Apr 22;10(4):646. doi: 10.3390/biom10040646.
For the first time, 3D chitin scaffolds from the marine demosponge were used for adsorption and immobilization of laccase from . The resulting chitin-enzyme biocatalytic systems were applied in the removal of tetracycline. Effective enzyme immobilization was confirmed by scanning electron microscopy. Immobilization yield and kinetic parameters were investigated in detail, in addition to the activity of the enzyme after immobilization. The designed systems were further used for the removal of tetracycline under various process conditions. Optimum process conditions, enabling total removal of tetracycline from solutions at concentrations up to 1 mg/L, were found to be pH 5, temperature between 25 and 35 °C, and 1 h process duration. Due to the protective effect of the chitinous scaffolds and stabilization of the enzyme by multipoint attachment, the storage stability and thermal stability of the immobilized biomolecules were significantly improved as compared to the free enzyme. The produced biocatalytic systems also exhibited good reusability, as after 10 repeated uses they removed over 90% of tetracycline from solution. Finally, the immobilized laccase was used in a packed bed reactor for continuous removal of tetracycline, and enabled the removal of over 80% of the antibiotic after 24 h of continuous use.
首次使用来自海洋寻常海绵的 3D 几丁质支架来吸附和固定 中的漆酶。所得的几丁质-酶生物催化体系被应用于去除四环素。通过扫描电子显微镜证实了有效的酶固定化。除了固定化酶的活性外，还详细研究了固定化产率和动力学参数。进一步在各种工艺条件下使用设计的系统去除四环素。发现最佳工艺条件为 pH5、温度在 25 到 35°C 之间、处理时间为 1 小时，可使高达 1mg/L 浓度的四环素溶液中的四环素完全去除。由于几丁质支架的保护作用和酶的多点附着稳定作用，与游离酶相比，固定化生物分子的储存稳定性和热稳定性显著提高。所制备的生物催化体系还表现出良好的可重复使用性，经过 10 次重复使用后，从溶液中去除了超过 90%的四环素。最后，将固定化漆酶用于填充床反应器中连续去除四环素，连续使用 24 小时后，抗生素的去除率超过 80%。
