School Bio-Sciences and Technology, Vellore Institute of Technology, Vellore 632014, Tamil Nadu, India.
School Bio-Sciences and Technology, Vellore Institute of Technology, Vellore 632014, Tamil Nadu, India.
Int Immunopharmacol. 2020 Mar;80:106136. doi: 10.1016/j.intimp.2019.106136. Epub 2020 Jan 25.
Garlic (Allium sativum) - derived organosulfur compound diallyl disulfide (DADS) possesses antioxidant, anti-inflammatory and anti-cancer effects. This study was aimed to investigate the anti-inflammatory role and the underlying molecular mechanisms of DADS in cerulein-induced acute pancreatitis (AP) and associated lung injury. Administration of DADS significantly attenuated the severity of pancreatic and pulmonary inflammation by inhibiting cerulein induced serum amylase, myeloperoxidase activity (MPO) and histological changes in pancreas and lung. Furthermore, the anti-inflammatory effect of DADS was associated with the decrease in tumor necrosis factor (TNF)-α,cystathionine-γ-lyase (CSE), preprotachykinin A (PPTA), neurokinin-1-receptor (NK1R) expression and hydrogen sulfide (HS) production in both pancreas and lung. In addition, DADS reduced caerulein-induced I-κB degradation and subsequent translocation of NF-κB in the pancreas and lung. These results show for the first time that in AP, DADS exhibits an anti-inflammatory effect by inhibiting CSE/HS and SP/NK1R signaling and NF-кB pathway.
大蒜（Allium sativum）衍生的有机硫化合物二烯丙基二硫（DADS）具有抗氧化、抗炎和抗癌作用。本研究旨在探讨 DADS 在鹅膏蕈碱诱导的急性胰腺炎（AP）及相关肺损伤中的抗炎作用及其潜在的分子机制。DADS 的给药显著通过抑制鹅膏蕈碱诱导的血清淀粉酶、髓过氧化物酶活性（MPO）和胰腺和肺组织学变化，减轻胰腺和肺的炎症严重程度。此外，DADS 的抗炎作用与胰腺和肺中肿瘤坏死因子（TNF）-α、胱硫醚-γ-裂解酶（CSE）、前原脑啡肽 A（PPTA）、神经激肽-1 受体（NK1R）表达和硫化氢（HS）产生的减少有关。此外，DADS 减少了鹅膏蕈碱诱导的胰腺和肺中 I-κB 的降解以及随后 NF-κB 的易位。这些结果首次表明，在 AP 中，DADS 通过抑制 CSE/HS 和 SP/NK1R 信号转导和 NF-κB 通路发挥抗炎作用。
