State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, P. R. China.
Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, State Key Laboratory Breeding Base of Marine Genetic Resources, Fujian Key Laboratory of Marine Genetic Resources, Xiamen, 361005, P. R. China.
J Microbiol. 2020 Feb;58(2):92-98. doi: 10.1007/s12275-020-9405-z. Epub 2020 Jan 29.
A Gram-stain-negative strictly aerobic, short-rod-shaped, and non-motile bacterial strain designated HSLHS9 was isolated from surface seawater collected from the South China Sea. Strain HSLHS9 could grow at 15-41°C (optimum 28°C), at pH 5.0-9.0 (optimum 6.0-7.0), and in 0-7% (w/v) NaCl (optimum 2-3%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HSLHS9 shared high identities with the closely related Parahaliea aestuarii S2-26 (98.6%) and Parahaliea mediterranea 7SM29 (97.8%) and formed a distinct lineage within the genus Parahaliea. Wholegenome sequencing of strain HSLHS9 revealed the size of 4.8 Mbp and DNA G + C content of 61.8 mol%. Strain HSLHS9 shared the digital DNA-DNA hybridization values of 22.4% and 23.0%, and the average nucleotide identities of 79.7% and 79.9%, respectively, with the two type strains above. The predominant cellular fatty acids of the strain were summed feature 8 (Cω6c and/or Cω7c), summed feature 3 (Cω7c and/or Cω6c), Cω8c, and C. The sole isoprenoid quinone was identified as Q-8. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, aminolipid, and two glycolipids. Based on taxonomic data obtained in this study, it is suggested that strain HSLHS9 represents a novel species of the genus Parahaliea, for which the name Parahaliea maris sp. nov. is proposed. The type strain is HSLHS9 (= MCCC 1A06717 = KCTC 52307). An emended description of the genus Parahaliea is also provided.
一株革兰氏阴性、严格需氧、短杆状、非运动的细菌菌株 HSLHS9 从南海采集的表层海水中分离得到。菌株 HSLHS9 可在 15-41°C（最佳 28°C）、pH5.0-9.0（最佳 6.0-7.0）和 0-7%（w/v）NaCl（最佳 2-3%）的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 HSLHS9 与密切相关的 Parahaliea aestuarii S2-26（98.6%）和 Parahaliea mediterranea 7SM29（97.8%）具有较高的同源性，并在 Parahaliea 属内形成一个独特的分支。菌株 HSLHS9 的全基因组测序显示其大小为 4.8 Mbp，DNA G+C 含量为 61.8 mol%。菌株 HSLHS9 与上述两种模式菌株的数字 DNA-DNA 杂交值分别为 22.4%和 23.0%，平均核苷酸同一性分别为 79.7%和 79.9%。该菌株的主要细胞脂肪酸为 sum feature 8（Cω6c 和/或 Cω7c）、sum feature 3（Cω7c 和/或 Cω6c）、Cω8c 和 C。唯一的异戊二烯醌为 Q-8。极性脂为磷脂酰甘油、双磷脂酰甘油、磷脂酰乙醇胺、胺脂和两种糖脂。根据本研究获得的分类学数据，建议菌株 HSLHS9 代表 Parahaliea 属的一个新种，命名为 Parahaliea maris sp. nov。模式菌株为 HSLHS9（=MCCC 1A06717=KCTC 52307）。还提供了 Parahaliea 属的修订描述。
