Xu Lin, Wu Yue-Hong, Jian Shu-Ling, Wang Chun-Sheng, Wu Min, Cheng Lei, Xu Xue-Wei
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
Int J Syst Evol Microbiol. 2016 Dec;66(12):5155-5160. doi: 10.1099/ijsem.0.001489. Epub 2016 Sep 9.
Two Gram-stain-negative, aerobic, motile by a single polar flagellum and rod-shaped strains, designated SCS-49T and SCS-111, were isolated from seawater of the South China Sea. The two strains grew at 4-35 °C, with 0.5-7.5 % (w/v) NaCl and at pH 6.5-9.0 and were able to reduce nitrate. Q-8 was the sole ubiquinone. The major fatty acids of the two strains were C16 : 0, C18 : 1ω7c and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospoglycolipid, three unidentified glycolipids, five unidentified phospholipids and two to three unidentified lipids. The isolates formed a stable clade with Pseudohongiella acticola and Pseudohongiella spirulinae based on phylogenetic analysis of 16S rRNA gene sequences. Strains SCS-49T and SCS-111 exhibited 16S rRNA gene sequence similarity values of 97.2 and 96.0 % with respect to the type strains of P. acticola and P. spirulinae, respectively. The average nucleotide diversity and in silico DNA-DNA hybridization values between strain SCS-49T and P. acticola KCTC 42131T were 71.4 and 25.1 %, respectively and the values between strain SCS-49T and SCS-111 were 99.9 and 99.2 %, respectively. Based upon the phenotypic, chemotaxonomic and genetic data, strains SCS-49T and SCS-111 represent a novel species in the genus Pseudohongiella, for which the name Pseudohongiella nitratireducens sp. nov. is proposed. The type strain is SCS-49T (=CGMCC 1.15425T=KCTC 52155T=MCCC 1K03186T).
从中国南海海水中分离出两株革兰氏阴性、需氧、具单极鞭毛且呈杆状的菌株，分别命名为SCS-49T和SCS-111。这两株菌在4-35 °C、含0.5-7.5 %（w/v）NaCl以及pH 6.5-9.0的条件下生长，且能够还原硝酸盐。泛醌Q-8是唯一的泛醌。这两株菌的主要脂肪酸为C16 : 0、C18 : 1ω7c以及特征性总和3（C16 : 1ω7c和/或C16 : 1ω6c）。极性脂类包括双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷酸糖脂、三种未鉴定的糖脂、五种未鉴定的磷脂以及两到三种未鉴定的脂类。基于16S rRNA基因序列的系统发育分析，分离株与海生拟红球菌和螺旋拟红球菌形成一个稳定的分支。菌株SCS-49T和SCS-111与海生拟红球菌和螺旋拟红球菌模式菌株的16S rRNA基因序列相似性值分别为97.2%和96.0%。菌株SCS-49T与海生拟红球菌KCTC 42131T之间的平均核苷酸多样性和电子计算机DNA-DNA杂交值分别为71.4%和25.1%，菌株SCS-49T与SCS-111之间的相应值分别为99.9%和99.2%。基于表型、化学分类学和遗传学数据，菌株SCS-49T和SCS-111代表拟红球菌属中的一个新物种，为此提出新名称硝酸盐还原拟红球菌（Pseudohongiella nitratireducens sp. nov.）。模式菌株为SCS-49T（=CGMCC 1.15425T=KCTC 52155T=MCCC 1K03186T）。
