Yang Pengyan, Yang Wenhui, Zhang Haiyang, Zhao Rui
School of Light Industry, Beijing Technology and Business University (BTBU), Beijing 100048, China.
Department of Biological Science and Engineering, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Materials (Basel). 2023 Oct 6;16(19):6572. doi: 10.3390/ma16196572.
Oxidoreductases are a wide class of enzymes that can catalyze biological oxidation and reduction reactions. Nowadays, oxidoreductases play a vital part in most bioenergetic metabolic pathways, which have important applications in biodegradation, bioremediation, environmental applications, as well as biosensors. However, free oxidoreductases are not stable and hard to be recycled. In addition, cofactors are needed in most oxidoreductases catalyze reactions, which are so expensive and unstable that it hinders their industrial applications. Enzyme immobilization is a feasible strategy that can overcome these problems. Recently, metal-organic frameworks (MOFs) have shown great potential as support materials for immobilizing enzymes due to their unique properties, such as high surface-area-to-volume ratio, chemical stability, functional designability, and tunable pore size. This review discussed the application of MOFs and their composites as immobilized carriers of oxidoreductase, as well as the application of MOFs as catalysts and immobilized carriers in redox reactions in the perspective of the function of MOFs materials. The paper also focuses on the potential of MOF carrier-based oxidoreductase immobilization for designing an enzyme cascade reaction system.
氧化还原酶是一类广泛的酶，能够催化生物氧化和还原反应。如今，氧化还原酶在大多数生物能量代谢途径中发挥着至关重要的作用，这些途径在生物降解、生物修复、环境应用以及生物传感器方面都有重要应用。然而，游离的氧化还原酶不稳定且难以循环利用。此外，大多数氧化还原酶催化反应都需要辅因子，这些辅因子既昂贵又不稳定，这阻碍了它们的工业应用。酶固定化是一种可以克服这些问题的可行策略。近年来，金属有机框架（MOF）由于其独特的性质，如高的比表面积、化学稳定性、功能可设计性和可调孔径，作为固定化酶的载体材料显示出巨大潜力。本综述从MOF材料的功能角度讨论了MOF及其复合材料作为氧化还原酶固定化载体的应用，以及MOF作为催化剂和固定化载体在氧化还原反应中的应用。本文还重点探讨了基于MOF载体的氧化还原酶固定化在设计酶级联反应系统方面的潜力。
