基质金属蛋白酶和 miRNA 在口腔鳞状细胞癌转移中的差异表达。
Differential expression of matrix metalloproteinases and miRNAs in the metastasis of oral squamous cell carcinoma.
机构信息
Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 639 Zhizaoju Road, Shanghai, 200011, China.
出版信息
BMC Oral Health. 2020 Jan 29;20(1):24. doi: 10.1186/s12903-020-1013-0.
BACKGROUND
Our study aimed to reveal the regulatory mechanisms of miRNAs and matrix metalloproteinases (MMPs) in oral squamous cell carcinoma (OSCC).
METHODS
The mRNA and miRNA expression profiles of six metastatic tumour samples, six nonmetastatic tumour samples, and six normal tissue samples were used for microarray analysis. Moreover, the important genes and miRNAs were validated by published profiles in Oncomine and by qRT-PCR.
RESULTS
MMP7, MMP13, and MMP10 were upregulated, and MMP12 and MMP9 were downregulated in metastatic tumours compared with nonmetastatic tumours. MMP7 was regulated by miR-4697-5p and miR-7109-5p. MMP7 and MMP13 were upregulated in OSCC samples compared with normal samples in Oncomine. Moreover, qRT-PCR revealed that the expression of miR-7109-5p and miR-34b was decreased in metastatic tumours compared with nonmetastatic tumours.
CONCLUSIONS
Our study suggested that miR-7109-5p and miR-34b might play important roles in the metastasis of OSCC by regulating MMP7 and MMP13 expression, respectively.
背景
本研究旨在揭示 miRNA 和基质金属蛋白酶（MMPs）在口腔鳞状细胞癌（OSCC）中的调控机制。
方法
使用微阵列分析了 6 个转移性肿瘤样本、6 个非转移性肿瘤样本和 6 个正常组织样本的 mRNA 和 miRNA 表达谱。此外，通过 Oncomine 中的已发表图谱和 qRT-PCR 验证了重要基因和 miRNA。
结果
与非转移性肿瘤相比，转移性肿瘤中 MMP7、MMP13 和 MMP10 上调，而 MMP12 和 MMP9 下调。MMP7 受 miR-4697-5p 和 miR-7109-5p 调控。在 Oncomine 中，与正常样本相比，OSCC 样本中 MMP7 和 MMP13 的表达上调。此外，qRT-PCR 显示转移性肿瘤中 miR-7109-5p 和 miR-34b 的表达较非转移性肿瘤降低。
结论
本研究表明，miR-7109-5p 和 miR-34b 可能通过调节 MMP7 和 MMP13 的表达，分别在 OSCC 的转移中发挥重要作用。
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