Savita J K, Varsha V K, Girish H C
Department of Oral and Maxillofacial Pathology, Rajarajeswari Dental College and Hospital, Bengaluru, Karnataka, India.
J Oral Maxillofac Pathol. 2023 Apr-Jun;27(2):315-322. doi: 10.4103/jomfp.jomfp_438_22. Epub 2023 Jul 13.
Matrix metalloproteinases (MMPs) are basically a part of a large family of proteolytic enzymes. They play an important role in degrading extracellular matrix and basement membrane, which is a basic mechanism in local invasion and tumour metastasis. The aim of this study was to evaluate immunohistochemically the expression of MMP1 and MMP10 in tumour invasion locally and at distant levels, including lymph nodes at different levels in oral squamous cell carcinoma (OSCC).
A total of 50 tissue samples with clinically confirmed OSCC and 15 normal oral mucosal tissues will be included in the study. Immunohistochemical staining will be performed for the demonstration of MMP1 and MMP10 in lesional tissue, perilesional tissue, and lymph nodes of different levels that were evaluated with respect to microscopic features.
All OSCC cases had MMP1 and MMP10 expression levels. The expression increased as the nodal level increased from level I to level V. This difference was statistically significant at < 0.001 Both MMPs were not expressed in normal epithelial cells. There was no significant correlation between MMP1 and MMP10 expression.
This study showed that MMP1 and MMP10 are expressed in the tissues of OSCC and may serve as prognostic indicators for the disease.
基质金属蛋白酶（MMPs）基本上是一个蛋白水解酶大家族的一部分。它们在降解细胞外基质和基底膜中发挥重要作用，这是局部侵袭和肿瘤转移的基本机制。本研究的目的是通过免疫组织化学方法评估MMP1和MMP10在口腔鳞状细胞癌（OSCC）局部和远处包括不同水平淋巴结中的肿瘤侵袭表达情况。
本研究将纳入50例临床确诊为OSCC的组织样本和15例正常口腔黏膜组织。将对病变组织、病变周围组织以及根据微观特征评估的不同水平淋巴结进行免疫组织化学染色，以显示MMP1和MMP10。
所有OSCC病例均有MMP1和MMP10表达水平。随着淋巴结水平从I级增加到V级，表达增加。这种差异在<0.001时有统计学意义。两种MMPs在正常上皮细胞中均不表达。MMP1和MMP10表达之间无显著相关性。
本研究表明，MMP1和MMP10在OSCC组织中表达，可能作为该疾病的预后指标。
