Graduate School of Biochemical Engineering, Ming Chi University of Technology, New Taipei City, Taiwan.
Phytochem Anal. 2020 Sep;31(5):575-582. doi: 10.1002/pca.2922. Epub 2020 Jan 29.
Rosmarinic acid is a bioactive compound with various pharmaceutical effects and applications.
This work developed a new approach for aqueous enzymatic extraction of rosmarinic acid from the leaves of Salvia officinalis.
Different enzymes (proteases and cellulase) were evaluated for their extraction activity. Response surface methodology (RSM) was subsequently employed to optimise the extraction conditions. Thin layer chromatography was also used to identify rosmarinic acid in the extract of S. officinalis.
Among the tested enzymes, a Cellulase A and Protamex mixture (1:1, w/w) exhibited maximum effectiveness in the extraction. Through the use of RSM, the maximum rosmarinic acid content of 28.23 ± 0.41 mg/g was obtained with enzyme loading of 4.49%, water-to-sample ratio of 25.76 mL/g, temperature of 54.3°C, and extraction time of 2 h.
This study suggests that S. officinalis is a promising source of rosmarinic acid and aqueous enzymatic extraction is an efficient and ecofriendly method for extracting rosmarinic acid, with a short extraction time and without the contamination of a toxic solvent.
迷迭香酸是一种具有多种药用功效和应用的生物活性化合物。
本工作开发了一种从药用鼠尾草叶片中酶法水相提取迷迭香酸的新方法。
评估了不同的酶（蛋白酶和纤维素酶）的提取活性。随后采用响应面法（RSM）优化提取条件。还使用薄层层析法鉴定了 S. officinalis 提取物中的迷迭香酸。
在所测试的酶中，纤维素酶 A 和 Protamex 混合物（1:1，w/w）在提取中表现出最大的有效性。通过使用 RSM，在酶用量为 4.49%、水与样品比为 25.76 mL/g、温度为 54.3°C、提取时间为 2 h 的条件下，迷迭香酸的最大含量为 28.23±0.41 mg/g。
本研究表明，药用鼠尾草是迷迭香酸的一个很有前途的来源，水相酶法提取是一种高效、环保的提取迷迭香酸的方法，具有提取时间短、无有毒溶剂污染的优点。
