Mousavi Monireh-Sadat, Imani Alireza, Meknatkhah Sogol, Riazi Gholamhossein
Laboratory of Neuro-Organic Chemistry, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Basic Med Sci. 2019 Oct;22(10):1179-1185. doi: 10.22038/ijbms.2019.32888.7857.
Association of adolescent emotional stress (ES) with the incidence of cardiovascular disease (CVD) at older age was investigated.
21 female rats were divided into three groups of 7 each; ES, foot-shock, and control. Chronic ES was induced by exposing the rats to witness foot-shock of their neighboring counterparts in the stress-box system in 5 successive days. 6 weeks after the last stress exposure, M-Mode echocardiographic assessment, qRT-PCR, and western blotting were performed in adult rats to determine the persistent effect of adolescent ES on cardiac performance and gene/protein expression levels of cardiac natriuretic peptide receptor 3 (NPR3) as a biomarker of CVD.
Interventricular septum thicknesses in diastole (IVSd) increased from 0.152±0.007 cm to 0.197±0.016 cm (<0.05), left ventricular posterior wall thickness in diastole (LVPWd) significantly enlarged from 0.169±0.006 cm to 0.288±0.033 cm (<0.01), left ventricular posterior wall thickness in systole (LVPWs) enlarged from 0.223±0.012 cm to 0.318±0.038 cm (<0.05), left ventricular mass increased from 1.000±0.024 g to 1.283±0.084 g (<0.01), and mean heart rate elevated from 229.42±6.57 bpm to 280.29±10.45 bpm (<0.01). Moreover, ES significantly upregulated the expression levels of cardiac NPR3 gene (<0.01) and protein (<0.01).
The incidence of adult CVD seemed to be increased under the influence of adolescent ES. Consequently, we suggest that mental healthcare during adolescence would be a critical factor for adult CVD prevention.
研究青少年情绪应激（ES）与老年心血管疾病（CVD）发病率之间的关联。
将21只雌性大鼠分为三组，每组7只；分别为情绪应激组、足部电击组和对照组。通过在应激箱系统中让大鼠连续5天目睹相邻同伴遭受足部电击来诱导慢性情绪应激。在最后一次应激暴露6周后，对成年大鼠进行M型超声心动图评估、qRT-PCR和蛋白质印迹分析，以确定青少年情绪应激对心脏功能以及作为心血管疾病生物标志物的心脏利钠肽受体3（NPR3）基因/蛋白质表达水平的持续影响。
舒张期室间隔厚度（IVSd）从0.152±0.007厘米增加到0.197±0.016厘米（<0.05），舒张期左心室后壁厚度（LVPWd）从0.169±0.006厘米显著增大到0.288±0.033厘米（<0.01），收缩期左心室后壁厚度（LVPWs）从0.223±0.012厘米增大到0.318±0.038厘米（<0.05），左心室质量从1.000±0.024克增加到1.283±0.084克（<0.01），平均心率从229.42±6.57次/分钟升高到280.29±10.45次/分钟（<0.01）。此外，情绪应激显著上调了心脏NPR3基因（<0.01）和蛋白质（<0.01）的表达水平。
在青少年情绪应激的影响下，成年心血管疾病的发病率似乎会增加。因此，我们认为青少年时期的心理保健将是预防成年心血管疾病的关键因素。
