Zhou Juan, Lei Jian, Wang Jun, Lian Chen-Lu, Hua Li, He Zhen-Yu, Wu San-Gang
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, Teaching Hospital of Fujian Medical University, Xiamen, China.
Department of Radiation Oncology, Cancer Hospital, The First Affiliated Hospital of Xiamen University, Teaching Hospital of Fujian Medical University, Xiamen, China.
Front Cell Dev Biol. 2020 Jan 9;7:361. doi: 10.3389/fcell.2019.00361. eCollection 2019.
Chemokine-like factor (CKLF)-like transmembrane members () represent a novel protein family linking the chemokine and transmembrane-4 superfamily families, which potentially play several roles in diverse physiological and pathological processes. The detailed functions and underlying molecular mechanisms of remain elusive in breast cancer. Herein, we performed a comprehensive bioinformatic analysis to investigate the prognostic effect, potential functions, and biomolecular regulatory network of in breast cancer. The mRNA expression level of , in particular, was significantly downregulated in breast cancer; moreover, high mRNA expression level of was significantly associated with better relapse-free survival. DNA promoter hypermethylation of was negatively correlated with its mRNA expression levels. Furthermore, strongly associated with pathway in MARVEL domains, chemotaxis, cytokines, transmembrane structures, and integral component of membrane. For example, genes related to MARVEL domains, transmembrane structures, and chemokines were significantly enriched. Our findings indicate that can be used as a prognostic biomarker and potential therapeutic target for breast cancer.
趋化因子样因子（CKLF）样跨膜成员（）代表了一个连接趋化因子和跨膜4超家族的新型蛋白质家族，其可能在多种生理和病理过程中发挥多种作用。在乳腺癌中，其详细功能和潜在分子机制仍不清楚。在此，我们进行了全面的生物信息学分析，以研究其在乳腺癌中的预后作用、潜在功能和生物分子调控网络。特别是，其mRNA表达水平在乳腺癌中显著下调；此外，高mRNA表达水平与更好的无复发生存显著相关。其DNA启动子高甲基化与其mRNA表达水平呈负相关。此外，其与MARVEL结构域、趋化作用、细胞因子、跨膜结构和膜整合成分中的通路密切相关。例如，与MARVEL结构域、跨膜结构和趋化因子相关的基因显著富集。我们的研究结果表明，其可作为乳腺癌的预后生物标志物和潜在治疗靶点。
