鉴定一种导致人类严重肺炎的新型冠状病毒：一项描述性研究。

Identification of a novel coronavirus causing severe pneumonia in human: a descriptive study.

机构信息

National Health Commission of the People's Republic of China Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.

Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, National Clinical Research Center for Respiratory Diseases, China-Japan Friendship Hospital, Beijing 100029, China.

出版信息

Chin Med J (Engl). 2020 May 5;133(9):1015-1024. doi: 10.1097/CM9.0000000000000722.

DOI:10.1097/CM9.0000000000000722

PMID:32004165

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7147275/

Abstract

BACKGROUND

Human infections with zoonotic coronaviruses (CoVs), including severe acute respiratory syndrome (SARS)-CoV and Middle East respiratory syndrome (MERS)-CoV, have raised great public health concern globally. Here, we report a novel bat-origin CoV causing severe and fatal pneumonia in humans.

METHODS

We collected clinical data and bronchoalveolar lavage (BAL) specimens from five patients with severe pneumonia from Wuhan Jinyintan Hospital, Hubei province, China. Nucleic acids of the BAL were extracted and subjected to next-generation sequencing. Virus isolation was carried out, and maximum-likelihood phylogenetic trees were constructed.

RESULTS

Five patients hospitalized from December 18 to December 29, 2019 presented with fever, cough, and dyspnea accompanied by complications of acute respiratory distress syndrome. Chest radiography revealed diffuse opacities and consolidation. One of these patients died. Sequence results revealed the presence of a previously unknown β-CoV strain in all five patients, with 99.8% to 99.9% nucleotide identities among the isolates. These isolates showed 79.0% nucleotide identity with the sequence of SARS-CoV (GenBank NC_004718) and 51.8% identity with the sequence of MERS-CoV (GenBank NC_019843). The virus is phylogenetically closest to a bat SARS-like CoV (SL-ZC45, GenBank MG772933) with 87.6% to 87.7% nucleotide identity, but is in a separate clade. Moreover, these viruses have a single intact open reading frame gene 8, as a further indicator of bat-origin CoVs. However, the amino acid sequence of the tentative receptor-binding domain resembles that of SARS-CoV, indicating that these viruses might use the same receptor.

CONCLUSION

A novel bat-borne CoV was identified that is associated with severe and fatal respiratory disease in humans.

摘要

背景

包括严重急性呼吸综合征（SARS）-CoV 和中东呼吸综合征（MERS）-CoV 在内的人畜共患冠状病毒（CoVs）感染引起了全球公众的极大关注。在这里，我们报告了一种新型的蝙蝠源性 CoV，可导致人类发生严重且致命的肺炎。

方法

我们收集了来自中国湖北省武汉市金银潭医院的五名重症肺炎患者的临床数据和支气管肺泡灌洗液（BAL）标本。从 BAL 中提取核酸并进行下一代测序。进行病毒分离，并构建最大似然系统发育树。

结果

2019 年 12 月 18 日至 12 月 29 日住院的五名患者表现出发热、咳嗽和呼吸困难，并伴有急性呼吸窘迫综合征的并发症。胸部 X 光显示弥漫性混浊和实变。其中一名患者死亡。序列结果显示，所有五名患者均存在一种以前未知的β-CoV 株，分离株之间的核苷酸同一性为 99.8%至 99.9%。这些分离株与 SARS-CoV（GenBank NC_004718）的序列具有 79.0%的核苷酸同一性，与 MERS-CoV（GenBank NC_019843）的序列具有 51.8%的同一性。该病毒在系统发育上与蝙蝠 SARS 样 CoV（SL-ZC45，GenBank MG772933）最为接近，核苷酸同一性为 87.6%至 87.7%，但属于不同的分支。此外，这些病毒具有一个完整的单一开放阅读框基因 8，这进一步表明它们是源自蝙蝠的 CoVs。然而，推定的受体结合结构域的氨基酸序列与 SARS-CoV 相似，表明这些病毒可能使用相同的受体。