超快速循环用于多重细胞荧光成像。
Ultra-fast Cycling for Multiplexed Cellular Fluorescence Imaging.
机构信息
Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, 02114, USA.
Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA, 02114, USA.
出版信息
Angew Chem Int Ed Engl. 2020 Apr 20;59(17):6839-6846. doi: 10.1002/anie.201915153. Epub 2020 Mar 6.
Abstract
Rapid analysis of single and scant cell populations is essential in modern diagnostics, yet existing methods are often limited and slow. Herein, we describe an ultra-fast, highly efficient cycling method for the analysis of single cells based on unique linkers for tetrazine (Tz)/trans-cyclooctene (TCO)-mediated quenching. Surprisingly, the quenching reaction rates were more than 3 orders of magnitude faster (t <1 s) than predicted. This allowed multi-cycle staining and immune cell profiling within an hour, leveraging the accelerated kinetics to open new diagnostic possibilities for rapid cellular analyses.
摘要
快速分析单细胞和稀少细胞群体在现代诊断中至关重要，但现有的方法往往受到限制且速度较慢。在此，我们描述了一种基于四嗪（Tz）/反式环辛烯（TCO）介导的猝灭的独特连接物的用于单细胞分析的超快速、高效的循环方法。令人惊讶的是，猝灭反应速率比预期快 3 个数量级以上（t <1 s）。这使得在一小时内可以进行多次循环染色和免疫细胞分析，利用加速动力学为快速细胞分析开辟了新的诊断可能性。
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