Department of Neurosurgery, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
Program for Computational and Systems Biology, Sloan Kettering Institute, New York, NY 10065, USA; New York Genome Center, New York, NY 10013, USA.
Stem Cell Reports. 2020 Feb 11;14(2):338-350. doi: 10.1016/j.stemcr.2020.01.007. Epub 2020 Jan 30.
Radial glia (RG) cells are the first neural stem cells to appear during embryonic development. Adult human glioblastomas harbor a subpopulation of RG-like cells with typical RG morphology and markers. The cells exhibit the classic and unique mitotic behavior of normal RG in a cell-autonomous manner. Single-cell RNA sequencing analyses of glioblastoma cells reveal transcriptionally dynamic clusters of RG-like cells that share the profiles of normal human fetal radial glia and that reside in quiescent and cycling states. Functional assays show a role for interleukin in triggering exit from dormancy into active cycling, suggesting a role for inflammation in tumor progression. These data are consistent with the possibility of persistence of RG into adulthood and their involvement in tumor initiation or maintenance. They also provide a putative cellular basis for the persistence of normal developmental programs in adult tumors.
放射状胶质细胞（RG）是胚胎发育过程中最早出现的神经干细胞。成人胶质母细胞瘤中存在一小部分 RG 样细胞，具有典型的 RG 形态和标志物。这些细胞以细胞自主性的方式表现出正常 RG 的典型和独特的有丝分裂行为。对胶质母细胞瘤细胞的单细胞 RNA 测序分析显示，RG 样细胞转录活跃簇具有正常人类胎儿放射状胶质细胞的特征，并处于静止和循环状态。功能分析表明白细胞介素在触发从休眠到活跃循环的转变中发挥作用，这表明炎症在肿瘤进展中起作用。这些数据与 RG 持续存在到成年期及其在肿瘤起始或维持中的参与的可能性一致。它们还为成人肿瘤中正常发育程序的持续存在提供了一个潜在的细胞基础。
