State Key Laboratory of Cotton Biology, School of Life Science, Henan University, Kaifeng, Henan, PR China; State Key Laboratory of Crop Stress Adaptation and Improvement, Henan University, Kaifeng, Henan, PR China.
State Key Laboratory of Cotton Biology, School of Life Science, Henan University, Kaifeng, Henan, PR China.
Plant Sci. 2020 Mar;292:110374. doi: 10.1016/j.plantsci.2019.110374. Epub 2019 Dec 13.
The soil-born vascular disease Verticillium wilt, which is caused by fungal pathogen Verticillium dahliae, is a devastating disease of cotton worldwide. In the last decade, a large number of genes have been found to participate in cotton-V. dahliae interactions, but the detailed mechanisms of cotton resistance to V. dahliae remain unclear. Here, we functionally characterized MPK3, a MAPK gene from cotton. MPK3 was induced in the roots of both resistant and susceptible cotton cultivars by V. dahliae inoculation. Transgenic cotton and tobacco with constitutively higher GbMPK3 expression conferred higher V. dahliae susceptibility, while MPK3 knockdown in cotton has limited effect on cotton resistance to V. dahliae. Expression profiling revealed that SA-mediated defense pathway genes (WRKY70, PR1, and PR5) accumulated after V. dahliae inoculation in roots of both wild-type and transgenic cotton, and the expression levels of these genes were higher in GbMPK3-overexpressing plants than in wild-type plants, indicating that GbMPK3 upregulation may reduce plant resistance to V. dahliae through regulating salicylic acid signaling transduction.
由真菌病原体黄萎轮枝菌引起的土传维管束病害黄萎病是一种世界性的棉花毁灭性病害。在过去的十年中，已经发现了大量参与棉花与黄萎轮枝菌互作的基因，但棉花对黄萎轮枝菌抗性的详细机制仍不清楚。在这里，我们对棉花中的一个 MAPK 基因 MPK3 进行了功能表征。黄萎轮枝菌接种后，抗性和感病棉花品种的根中均诱导了 MPK3 的表达。过表达 GbMPK3 的转基因棉花和烟草对黄萎轮枝菌的敏感性更高，而棉花中 MPK3 的敲低对棉花抗黄萎轮枝菌的能力影响有限。表达谱分析显示，黄萎轮枝菌接种后，野生型和转基因棉花根中 SA 介导的防御途径基因（WRKY70、PR1 和 PR5）积累，GbMPK3 过表达植株中这些基因的表达水平高于野生型植株，表明 GbMPK3 的上调可能通过调节水杨酸信号转导降低植物对黄萎轮枝菌的抗性。
